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1mh5

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[[Image:1mh5.jpg|left|200px]]<br /><applet load="1mh5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mh5, resolution 2.1&Aring;" />
 
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'''The Structure Of The Complex Of The Fab Fragment Of The Esterolytic Antibody MS6-164 and A Transition-State Analog'''<br />
 
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==Overview==
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==The Structure Of The Complex Of The Fab Fragment Of The Esterolytic Antibody MS6-164 and A Transition-State Analog==
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The crystal structures of four related Fab fragments of a family of, catalytic antibodies displaying differential levels of esterase activity, have been solved in the presence and in the absence of the, transition-state analogue (TSA) that was used to elicit the immune, response. The electron density maps show that the TSA conformation is, essentially identical, with limited changes on hapten binding., Interactions with the TSA explain the specificity for the D rather than, the L-isomer of the substrate. Differences in the residues in the, hapten-binding pocket, which increase hydrophobicity, appear to correlate, with an increase in the affinity of the antibodies for their substrate., Analysis of the structures at the active site reveals a network of, conserved hydrogen bond contacts between the TSA and the antibodies, and, points to a critical role of two conserved residues, HisL91 and LysH95, in, catalysis. However, these two key residues are set into very different, contexts in their respective structures, with an apparent direct, correlation between the catalytic power of the antibodies and the, complexity of their interactions with the rest of the protein. This, suggests that the catalytic efficiency may be controlled by contacts, arising from a second sphere of residues at the periphery of the active, site.
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<StructureSection load='1mh5' size='340' side='right'caption='[[1mh5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mh5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MH5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HAL:N-{[2-({[1-(4-CARBOXYBUTANOYL)AMINO]-2-PHENYLETHYL}-HYDROXYPHOSPHINYL)OXY]ACETYL}-2-PHENYLETHYLAMINE'>HAL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mh5 OCA], [https://pdbe.org/1mh5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mh5 RCSB], [https://www.ebi.ac.uk/pdbsum/1mh5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mh5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/1mh5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mh5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MH5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HAL:'>HAL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MH5 OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution crystal structure of the Fab-fragments of a family of mouse catalytic antibodies with esterase activity., Ruzheinikov SN, Muranova TA, Sedelnikova SE, Partridge LJ, Blackburn GM, Murray IA, Kakinuma H, Takahashi-Ando N, Shimazaki K, Sun J, Nishi Y, Rice DW, J Mol Biol. 2003 Sep 12;332(2):423-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12948492 12948492]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Blackburn GM]]
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[[Category: Blackburn, G.M.]]
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[[Category: Kakinuma H]]
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[[Category: Kakinuma, H.]]
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[[Category: Muranova TA]]
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[[Category: Muranova, T.A.]]
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[[Category: Murray IA]]
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[[Category: Murray, I.A.]]
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[[Category: Nishi Y]]
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[[Category: Nishi, Y.]]
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[[Category: Partridge LJ]]
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[[Category: Partridge, L.J.]]
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[[Category: Rice DW]]
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[[Category: Rice, D.W.]]
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[[Category: Ruzheinikov SN]]
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[[Category: Ruzheinikov, S.N.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S.E.]]
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[[Category: Shimazaki K]]
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[[Category: Shimazaki, K.]]
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[[Category: Sun J]]
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[[Category: Sun, J.]]
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[[Category: Takashi N]]
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[[Category: Takashi, N.]]
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[[Category: HAL]]
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[[Category: SO4]]
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[[Category: catalytic antibody]]
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[[Category: ester hydrolysis]]
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[[Category: esterolytic]]
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[[Category: fab]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:24:13 2008''
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Current revision

The Structure Of The Complex Of The Fab Fragment Of The Esterolytic Antibody MS6-164 and A Transition-State Analog

PDB ID 1mh5

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