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1mjj

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(New page: 200px<br /> <applet load="1mjj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjj, resolution 2.1&Aring;" /> '''HIGH RESOLUTION CRYS...)
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[[Image:1mjj.gif|left|200px]]<br />
 
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<applet load="1mjj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mjj, resolution 2.1&Aring;" />
 
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'''HIGH RESOLUTION CRYSTAL STRUCTURE OF THE COMPLEX OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12 AND A TRANSITION-STATE ANALOG'''<br />
 
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==Overview==
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==HIGH RESOLUTION CRYSTAL STRUCTURE OF THE COMPLEX OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12 AND A TRANSITION-STATE ANALOG==
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The crystal structures of four related Fab fragments of a family of, catalytic antibodies displaying differential levels of esterase activity, have been solved in the presence and in the absence of the, transition-state analogue (TSA) that was used to elicit the immune, response. The electron density maps show that the TSA conformation is, essentially identical, with limited changes on hapten binding., Interactions with the TSA explain the specificity for the D rather than, the L-isomer of the substrate. Differences in the residues in the, hapten-binding pocket, which increase hydrophobicity, appear to correlate, with an increase in the affinity of the antibodies for their substrate., Analysis of the structures at the active site reveals a network of, conserved hydrogen bond contacts between the TSA and the antibodies, and, points to a critical role of two conserved residues, HisL91 and LysH95, in, catalysis. However, these two key residues are set into very different, contexts in their respective structures, with an apparent direct, correlation between the catalytic power of the antibodies and the, complexity of their interactions with the rest of the protein. This, suggests that the catalytic efficiency may be controlled by contacts, arising from a second sphere of residues at the periphery of the active, site.
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<StructureSection load='1mjj' size='340' side='right'caption='[[1mjj]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mjj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HAL:N-{[2-({[1-(4-CARBOXYBUTANOYL)AMINO]-2-PHENYLETHYL}-HYDROXYPHOSPHINYL)OXY]ACETYL}-2-PHENYLETHYLAMINE'>HAL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjj OCA], [https://pdbe.org/1mjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjj RCSB], [https://www.ebi.ac.uk/pdbsum/1mjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjj ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MJJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 and HAL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MJJ OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution crystal structure of the Fab-fragments of a family of mouse catalytic antibodies with esterase activity., Ruzheinikov SN, Muranova TA, Sedelnikova SE, Partridge LJ, Blackburn GM, Murray IA, Kakinuma H, Takahashi-Ando N, Shimazaki K, Sun J, Nishi Y, Rice DW, J Mol Biol. 2003 Sep 12;332(2):423-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12948492 12948492]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Blackburn GM]]
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[[Category: Blackburn, G.M.]]
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[[Category: Kakinuma H]]
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[[Category: Kakinuma, H.]]
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[[Category: Muranova TA]]
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[[Category: Muranova, T.A.]]
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[[Category: Murray IA]]
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[[Category: Murray, I.A.]]
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[[Category: Nishi Y]]
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[[Category: Nishi, Y.]]
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[[Category: Partridge LJ]]
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[[Category: Partridge, L.J.]]
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[[Category: Rice DW]]
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[[Category: Rice, D.W.]]
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[[Category: Ruzheinikov SN]]
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[[Category: Ruzheinikov, S.N.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S.E.]]
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[[Category: Shimazaki K]]
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[[Category: Shimazaki, K.]]
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[[Category: Sun J]]
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[[Category: Sun, J.]]
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[[Category: Takashi N]]
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[[Category: Takashi, N.]]
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[[Category: HAL]]
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[[Category: SO4]]
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[[Category: catalytic antibody]]
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[[Category: ester hydrolysis]]
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[[Category: esterolytic]]
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[[Category: fab]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:36:56 2007''
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Current revision

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE COMPLEX OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12 AND A TRANSITION-STATE ANALOG

PDB ID 1mjj

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