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1mjn

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(New page: 200px<br /> <applet load="1mjn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mjn, resolution 1.30&Aring;" /> '''Crystal Structure o...)
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[[Image:1mjn.gif|left|200px]]<br />
 
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<applet load="1mjn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mjn, resolution 1.30&Aring;" />
 
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'''Crystal Structure of the intermediate affinity aL I domain mutant'''<br />
 
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==Overview==
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==Crystal Structure of the intermediate affinity aL I domain mutant==
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The structure of the I domain of integrin alpha L beta 2 bound to the Ig, superfamily ligand ICAM-1 reveals the open ligand binding conformation and, the first example of an integrin-IgSF interface. The I domain Mg2+, directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain, residue Glu-241 enables a critical salt bridge. Liganded and unliganded, structures for both high- and intermediate-affinity mutant I domains, reveal that ligand binding can induce conformational change in the alpha L, I domain and that allosteric signals can convert the closed conformation, to intermediate or open conformations without ligand binding. Pulling down, on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets, the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in, affinity.
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<StructureSection load='1mjn' size='340' side='right'caption='[[1mjn]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjn OCA], [https://pdbe.org/1mjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mjn RCSB], [https://www.ebi.ac.uk/pdbsum/1mjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mjn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ITAL_HUMAN ITAL_HUMAN] Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mjn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mjn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MJN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MJN OCA].
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*[[Integrin 3D structures|Integrin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation., Shimaoka M, Xiao T, Liu JH, Yang Y, Dong Y, Jun CD, McCormack A, Zhang R, Joachimiak A, Takagi J, Wang JH, Springer TA, Cell. 2003 Jan 10;112(1):99-111. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12526797 12526797]
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[[Category: Homo sapiens]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dong YC]]
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[[Category: Dong, Y.C.]]
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[[Category: Jun CD]]
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[[Category: Jun, C.D.]]
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[[Category: Liu JH]]
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[[Category: Liu, J.H.]]
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[[Category: McCormack A]]
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[[Category: McCormack, A.]]
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[[Category: Shimaoka M]]
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[[Category: Shimaoka, M.]]
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[[Category: Springer TA]]
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[[Category: Springer, T.A.]]
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[[Category: Wang JH]]
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[[Category: Wang, J.H.]]
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[[Category: Xiao T]]
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[[Category: Xiao, T.]]
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[[Category: Yang YT]]
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[[Category: Yang, Y.T.]]
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[[Category: Zhang RG]]
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[[Category: Zhang, R.G.]]
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[[Category: MG]]
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[[Category: rossmann fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:12:09 2007''
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Current revision

Crystal Structure of the intermediate affinity aL I domain mutant

PDB ID 1mjn

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