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1mky

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(New page: 200px<br /><applet load="1mky" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mky, resolution 1.90&Aring;" /> '''Structural Analysis ...)
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[[Image:1mky.jpg|left|200px]]<br /><applet load="1mky" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mky, resolution 1.90&Aring;" />
 
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'''Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains'''<br />
 
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==Overview==
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==Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains==
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The EngA subfamily of essential bacterial GTPases has a unique domain, structure consisting of two adjacent GTPase domains (GD1 and GD2) and a, C-terminal domain. The structure of Thermotoga maritima Der bound to GDP, determined at 1.9 A resolution reveals a novel domain arrangement in which, the GTPase domains pack at either side of the C-terminal domain., Unexpectedly, the C-terminal domain resembles a KH domain, missing the, distinctive RNA recognition elements. Conserved motifs of the nucleotide, binding site of GD1 are integral parts of the GD1-KH domain interface, suggesting the interactions between these two domains are directly, influenced by the GTP/GDP cycling of the protein. In contrast, the GD2-KH, domain interface is distal to the GDP binding site of GD2.
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<StructureSection load='1mky' size='340' side='right'caption='[[1mky]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MKY FirstGlance]. <br>
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1MKY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with PO4 and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MKY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mky OCA], [https://pdbe.org/1mky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mky RCSB], [https://www.ebi.ac.uk/pdbsum/1mky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mky ProSAT]</span></td></tr>
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Domain arrangement of Der, a switch protein containing two GTPase domains., Robinson VL, Hwang J, Fox E, Inouye M, Stock AM, Structure. 2002 Dec;10(12):1649-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12467572 12467572]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/DER_THEMA DER_THEMA] GTPase that plays an essential role in the late steps of ribosome biogenesis (By similarity). Has GTPase activity but no ATPase activity. GTP, GDP, and dGTP but not GMP, ATP, CTP, and UTP compete for GTP binding.[HAMAP-Rule:MF_00195]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mky_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mky ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Fox, E.]]
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[[Category: Fox E]]
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[[Category: Hwang, J.]]
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[[Category: Hwang J]]
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[[Category: Inouye, M.]]
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[[Category: Inouye M]]
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[[Category: Robinson, V.L.]]
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[[Category: Robinson VL]]
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[[Category: Stock, A.M.]]
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[[Category: Stock AM]]
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[[Category: GDP]]
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[[Category: PO4]]
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[[Category: der]]
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[[Category: enga]]
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[[Category: gtpase]]
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[[Category: kh-domain]]
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[[Category: tandem g-domains]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:29:21 2007''
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Current revision

Structural Analysis of the Domain Interactions in Der, a Switch Protein Containing Two GTPase Domains

PDB ID 1mky

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