This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1mn1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:38, 10 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mn1.jpg|left|200px]]
 
-
{{Structure
+
==MANGANESE PEROXIDASE SUBSTRATE BINDING SITE MUTANT D179N==
-
|PDB= 1mn1 |SIZE=350|CAPTION= <scene name='initialview01'>1mn1</scene>, resolution 2.0&Aring;
+
<StructureSection load='1mn1' size='340' side='right'caption='[[1mn1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
+
<table><tr><td colspan='2'>[[1mn1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MN1 FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Manganese_peroxidase Manganese peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.13 1.11.1.13]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
|GENE= MNP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5306 Phanerochaete chrysosporium])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mn1 OCA], [https://pdbe.org/1mn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mn1 RCSB], [https://www.ebi.ac.uk/pdbsum/1mn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mn1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PEM1_PHACH PEM1_PHACH] Catalyzes the oxidation of Mn(2+) to Mn(3+). The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mn1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mn1 ConSurf].
 +
<div style="clear:both"></div>
-
'''MANGANESE PEROXIDASE SUBSTRATE BINDING SITE MUTANT D179N'''
+
==See Also==
-
 
+
*[[Manganese peroxidase|Manganese peroxidase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Manganese peroxidase (MnP), an extracellular heme enzyme from the lignin-degrading basidiomycetous fungus, Phanerochaete chrysosporium, catalyzes the oxidation of MnII to MnIII. The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin model compounds. The proposed MnII binding site of MnP consists of a heme propionate, three acidic ligands (Glu-35, Glu-39, and Asp-179), and two water molecules. Using crystallographic methods, this binding site was probed by altering the amount of MnII bound to the protein. Crystals grown in the absence of MnII, or in the presence of EDTA, exhibited diminished electron density at this site. Crystals grown in excess MnII exhibited increased electron density at the proposed binding site but nowhere else in the protein. This suggests that there is only one major MnII binding site in MnP. Crystal structures of a single mutant (D179N) and a double mutant (E35Q,D179N) at this site were determined. The mutant structures lack a cation at the MnII binding site. The structure of the MnII binding site is altered significantly in both mutants, resulting in increased access to the solvent and substrate.
+
[[Category: Large Structures]]
-
 
+
[[Category: Phanerodontia chrysosporium]]
-
==About this Structure==
+
[[Category: Poulos TL]]
-
1MN1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MN1 OCA].
+
[[Category: Sundaramoorthy M]]
-
 
+
-
==Reference==
+
-
Crystal structures of substrate binding site mutants of manganese peroxidase., Sundaramoorthy M, Kishi K, Gold MH, Poulos TL, J Biol Chem. 1997 Jul 11;272(28):17574-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9211904 9211904]
+
-
[[Category: Manganese peroxidase]]
+
-
[[Category: Phanerochaete chrysosporium]]
+
-
[[Category: Single protein]]
+
-
[[Category: Poulos, T L.]]
+
-
[[Category: Sundaramoorthy, M.]]
+
-
[[Category: CA]]
+
-
[[Category: HEM]]
+
-
[[Category: donor: h2o2 oxidoreductase]]
+
-
[[Category: heme enzyme]]
+
-
[[Category: peroxidase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:45:30 2008''
+

Current revision

MANGANESE PEROXIDASE SUBSTRATE BINDING SITE MUTANT D179N

PDB ID 1mn1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mn1"
Personal tools