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1mpx

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(New page: 200px<br /><applet load="1mpx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mpx, resolution 1.90&Aring;" /> '''ALPHA-AMINO ACID EST...)
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[[Image:1mpx.gif|left|200px]]<br /><applet load="1mpx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mpx, resolution 1.90&Aring;" />
 
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'''ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE'''<br />
 
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==Overview==
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==ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE==
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alpha-Amino acid ester hydrolases (AEHs) catalyze the hydrolysis and, synthesis of esters and amides with an alpha-amino group. As such, they, can synthesize beta-lactam antibiotics from acyl compounds and beta-lactam, nuclei obtained from the hydrolysis of natural antibiotics. This article, describes the gene sequence and the 1.9-A resolution crystal structure of, the AEH from Xanthomonas citri. The enzyme consists of an, alpha/beta-hydrolase fold domain, a helical cap domain, and a jellyroll, beta-domain. Structural homology was observed to the Rhodococcus cocaine, esterase, indicating that both enzymes belong to the same class of, bacterial hydrolases. Docking of a beta-lactam antibiotic in the active, site explains the substrate specificity, specifically the necessity of an, alpha-amino group on the substrate, and explains the low specificity, toward the beta-lactam nucleus.
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<StructureSection load='1mpx' size='340' side='right'caption='[[1mpx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mpx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri Xanthomonas citri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPX FirstGlance]. <br>
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1MPX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_citri Xanthomonas citri] with CA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amino-acid_esterase Alpha-amino-acid esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.43 3.1.1.43] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MPX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpx OCA], [https://pdbe.org/1mpx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpx RCSB], [https://www.ebi.ac.uk/pdbsum/1mpx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpx ProSAT]</span></td></tr>
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The sequence and crystal structure of the alpha-amino acid ester hydrolase from Xanthomonas citri define a new family of beta-lactam antibiotic acylases., Barends TR, Polderman-Tijmes JJ, Jekel PA, Hensgens CM, de Vries EJ, Janssen DB, Dijkstra BW, J Biol Chem. 2003 Jun 20;278(25):23076-84. Epub 2003 Apr 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12684501 12684501]
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</table>
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[[Category: Alpha-amino-acid esterase]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q6YBS3_XANCI Q6YBS3_XANCI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpx ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Xanthomonas citri]]
[[Category: Xanthomonas citri]]
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[[Category: Barends, T.R.M.]]
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[[Category: Barends TRM]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Dijkstra BW]]
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[[Category: Hensgens, C.M.H.]]
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[[Category: Hensgens CMH]]
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[[Category: Janssen, D.B.]]
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[[Category: Janssen DB]]
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[[Category: Jekel, P.A.]]
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[[Category: Jekel PA]]
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[[Category: Polderman-Tijmes, J.J.]]
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[[Category: Polderman-Tijmes JJ]]
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[[Category: Vries, E.J.de.]]
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[[Category: De Vries EJ]]
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[[Category: CA]]
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[[Category: GOL]]
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[[Category: alpha/beta hydrolase]]
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[[Category: jellyroll]]
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[[Category: selenomethionine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:48:44 2007''
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Current revision

ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE

PDB ID 1mpx

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