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1msg

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(New page: 200px<br /> <applet load="1msg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1msg" /> '''SOLUTION STRUCTURE OF GRO(SLASH)MELANOMA GR...)
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[[Image:1msg.gif|left|200px]]<br />
 
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<applet load="1msg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1msg" />
 
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'''SOLUTION STRUCTURE OF GRO(SLASH)MELANOMA GROWTH STIMULATORY ACTIVITY DETERMINED BY 1H NMR SPECTROSCOPY'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF GRO(SLASH)MELANOMA GROWTH STIMULATORY ACTIVITY DETERMINED BY 1H NMR SPECTROSCOPY==
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The three-dimensional solution structure of the growth-related, protein-alpha/melanoma growth stimulatory activity (GRO/MGSA) has been, solved by two-dimensional 1H nuclear magnetic resonance spectroscopy. The, GRO/MGSA monomer consists of an NH2-terminal loop, a three-stranded, antiparallel beta-sheet, and a COOH-terminal alpha-helix. Dimerization, which is apparent under the experimental conditions used (2 mM, pH 5.10, 30 degrees C), results in a six-stranded antiparallel beta-sheet and a, pair of helices with 2-fold symmetry. While the basic fold is similar to, that seen for interleukin-8 (IL-8) (Clore, G. M., Appella, E., Yamada, M., Matsushima, K., and Gronenborn, A. M. (1990) Biochemistry, 29, 1689-1696), there are differences in the ELR motif (residues 6-8), the turn involving, residues 31-36, which is linked to the NH2-terminal region through the, 9-35 disulfide bond. The most significant differences are in the, NH2-terminal loop (residues 12-23). In IL-8, all the corresponding regions, have been shown to be required for receptor binding (Clark-Lewis, I., Dewald, B., Loetscher, M., Moser, B., and Baggiolini, M. (1994) J. Biol., Chem. 269, 16075-16081). The structural differences thus have been, identified between GRO/MGSA and IL-8 could contribute to their different, receptor binding specificities.
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<StructureSection load='1msg' size='340' side='right'caption='[[1msg]]' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1msg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MSG FirstGlance]. <br>
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Known diseases associated with this structure: AIDS, resistance to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600835 600835]], Osteogenesis imperfecta, type VIII OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=610339 610339]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1msg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1msg OCA], [https://pdbe.org/1msg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1msg RCSB], [https://www.ebi.ac.uk/pdbsum/1msg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1msg ProSAT]</span></td></tr>
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==About this Structure==
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</table>
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1MSG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MSG OCA].
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== Function ==
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[https://www.uniprot.org/uniprot/GROA_HUMAN GROA_HUMAN] Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.<ref>PMID:10095777</ref> <ref>PMID:2670560</ref>
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==Reference==
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== Evolutionary Conservation ==
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Solution structure of GRO/melanoma growth stimulatory activity determined by 1H NMR spectroscopy., Kim KS, Clark-Lewis I, Sykes BD, J Biol Chem. 1994 Dec 30;269(52):32909-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7806518 7806518]
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/1msg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1msg ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Clark-Lewis, I.]]
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[[Category: Clark-Lewis I]]
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[[Category: Kim, K.S.]]
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[[Category: Kim K-S]]
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[[Category: Sykes, B.D.]]
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[[Category: Sykes BD]]
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[[Category: cytokine (chemotactic)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:14:36 2007''
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Current revision

SOLUTION STRUCTURE OF GRO(SLASH)MELANOMA GROWTH STIMULATORY ACTIVITY DETERMINED BY 1H NMR SPECTROSCOPY

PDB ID 1msg

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