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1mtx

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(New page: 200px<br /><applet load="1mtx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mtx" /> '''DETERMINATION OF THE THREE-DIMENSIONAL STRUC...)
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[[Image:1mtx.jpg|left|200px]]<br /><applet load="1mtx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mtx" />
 
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'''DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''<br />
 
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==Overview==
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==DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY==
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The solution structure of the 39-residue peptide margatoxin, a scorpion, toxin that selectively blocks the voltage-gated potassium-channel Kv1.3, has been determined by NMR spectroscopy. The toxin was isotopically, labeled with 13C and 15N and studied using two-dimensional homonuclear and, three- and four-dimensional heteronuclear NMR spectroscopy. The final, structure was determined using 501 constraints, comprising 422 NOE, constraints, 60 dihedral angle constraints, 9 disulfide constraints, and, 10 hydrogen bond constraints. Structures were initially determined with, the program PEGASUS and subsequently refined with X-PLOR. The average rms, deviation from a calculated average structure for the backbone atoms of, residues 3-38 is 0.40 A. A helix is present from residues 11 to 20 and, includes two proline residues at positions 15 and 16. A loop at residues, 21-24 leads into a two-strand antiparallel sheet from residues 25 to 38, with a turn at residues 30-33. Residues 3-6 run adjacent to the 33-38, strand but do not form a canonical beta-strand. The two additional, residues of margatoxin, relative to the related toxins charybdotoxin and, iberiotoxin, insert in a manner that extends the beta-sheet by one, residue. Otherwise, the global structure is very similar to that of these, two other toxins. The longer sheet may have implications for channel, selectivity.
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<StructureSection load='1mtx' size='340' side='right'caption='[[1mtx]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MTX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mtx OCA], [https://pdbe.org/1mtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mtx RCSB], [https://www.ebi.ac.uk/pdbsum/1mtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mtx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KAX22_CENMA KAX22_CENMA] Potent selective inhibitor of voltage-dependent potassium channels such as Kv1.3/KCNA3.<ref>PMID:8360176</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/1mtx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mtx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_margaritatus Centruroides margaritatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MTX OCA].
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*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Determination of the three-dimensional structure of margatoxin by 1H, 13C, 15N triple-resonance nuclear magnetic resonance spectroscopy., Johnson BA, Stevens SP, Williamson JM, Biochemistry. 1994 Dec 20;33(50):15061-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7999764 7999764]
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__TOC__
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</StructureSection>
[[Category: Centruroides margaritatus]]
[[Category: Centruroides margaritatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Johnson, B.A.]]
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[[Category: Johnson BA]]
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[[Category: Stevens, S.P.]]
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[[Category: Stevens SP]]
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[[Category: Williamson, J.M.]]
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[[Category: Williamson JM]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:41:52 2007''
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Current revision

DETERMINATION OF THE THREE-DIMENSIONAL STRUCTURE OF MARGATOXIN BY 1H, 13C, 15N TRIPLE-RESONANCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

PDB ID 1mtx

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