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1muu

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(New page: 200px<br /><applet load="1muu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1muu, resolution 2.0&Aring;" /> '''2.0 A crystal structu...)
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[[Image:1muu.gif|left|200px]]<br /><applet load="1muu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1muu, resolution 2.0&Aring;" />
 
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'''2.0 A crystal structure of GDP-mannose dehydrogenase'''<br />
 
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==Overview==
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==2.0 A crystal structure of GDP-mannose dehydrogenase==
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The enzyme GMD from Pseudomonas aeruginosa catalyzes the committed step in, the synthesis of the exopolysaccharide alginate. Alginate is a major, component of P. aeruginosa biofilms that protect the bacteria from the, host immune response and antibiotic therapy. The 1.55 A crystal structure, of GMD in ternary complex with its cofactor NAD(H) and product, GDP-mannuronic acid reveals that the enzyme forms a domain-swapped dimer, with two polypeptide chains contributing to each active site. The, extensive dimer interface provides multiple opportunities for intersubunit, communication. Comparison of the GMD structure with that of UDP-glucose, dehydrogenase reveals the structural basis of sugar binding specificity, that distinguishes these two related enzyme families. The high-resolution, structure of GMD provides detailed information on the active site of the, enzyme and a template for structure-based inhibitor design.
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<StructureSection load='1muu' size='340' side='right'caption='[[1muu]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1muu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MUU FirstGlance]. <br>
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1MUU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with SUC, NAD and GDX as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/GDP-mannose_6-dehydrogenase GDP-mannose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.132 1.1.1.132] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GDX:GUANOSINE+5-(TRIHYDROGEN+DIPHOSPHATE),+P-D-MANNOPYRANOSYL+ESTER'>GDX</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1muu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1muu OCA], [https://pdbe.org/1muu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1muu RCSB], [https://www.ebi.ac.uk/pdbsum/1muu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1muu ProSAT]</span></td></tr>
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Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa., Snook CF, Tipton PA, Beamer LJ, Biochemistry. 2003 Apr 29;42(16):4658-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12705829 12705829]
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</table>
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[[Category: GDP-mannose 6-dehydrogenase]]
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== Function ==
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[https://www.uniprot.org/uniprot/ALGD_PSEAE ALGD_PSEAE] Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1muu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1muu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Beamer LJ]]
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[[Category: Beamer, L.J.]]
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[[Category: Snook CF]]
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[[Category: Snook, C.F.]]
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[[Category: Tipton PA]]
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[[Category: Tipton, P.A.]]
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[[Category: GDX]]
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[[Category: NAD]]
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[[Category: SUC]]
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[[Category: rossman fold; domain-swapped dimer; enzyme complex with cofactor and product]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:43:07 2007''
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Current revision

2.0 A crystal structure of GDP-mannose dehydrogenase

PDB ID 1muu

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