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1mwt

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(New page: 200px<br /><applet load="1mwt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mwt, resolution 2.45&Aring;" /> '''Structure of penicil...)
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[[Image:1mwt.jpg|left|200px]]<br /><applet load="1mwt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mwt, resolution 2.45&Aring;" />
 
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'''Structure of penicillin G acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.45 A resolution.'''<br />
 
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==Overview==
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==Structure of penicillin G acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.45 A resolution.==
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The multiple antibiotic resistance of methicillin-resistant strains of, Staphylococcus aureus (MRSA) has become a major clinical problem, worldwide. The key determinant of the broad-spectrum beta-lactam, resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a)., Because of its low affinity for beta-lactams, PBP2a provides, transpeptidase activity to allow cell wall synthesis at beta-lactam, concentrations that inhibit the beta-lactam-sensitive PBPs normally, produced by S. aureus. The crystal structure of a soluble derivative of, PBP2a has been determined to 1.8 A resolution and provides the highest, resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin, G and methicillin allow, for the first time, a comparison of an apo and, acylated resistant PBP. An analysis of the PBP2a active site in these, forms reveals the structural basis of its resistance and identifies, features in newly developed beta-lactams that are likely important for, high affinity binding.
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<StructureSection load='1mwt' size='340' side='right'caption='[[1mwt]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PNM:OPEN+FORM+-+PENICILLIN+G'>PNM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwt OCA], [https://pdbe.org/1mwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwt RCSB], [https://www.ebi.ac.uk/pdbsum/1mwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93IC2_STAAU Q93IC2_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MWT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CD and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MWT OCA].
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*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus., Lim D, Strynadka NC, Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12389036 12389036]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Lim, D.C.]]
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[[Category: Lim DC]]
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[[Category: Strynadka, N.C.J.]]
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[[Category: Strynadka NCJ]]
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[[Category: CD]]
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[[Category: CL]]
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[[Category: beta-lactam]]
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[[Category: d]]
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[[Category: d-carboxypeptidase]]
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[[Category: d-transpeptidase]]
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[[Category: penicillin binding protein]]
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[[Category: penicillin g]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:08:44 2007''
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Current revision

Structure of penicillin G acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.45 A resolution.

PDB ID 1mwt

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