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1nco

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(New page: 200px<br /><applet load="1nco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nco, resolution 1.8&Aring;" /> '''STRUCTURE OF THE ANTI...)
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[[Image:1nco.gif|left|200px]]<br /><applet load="1nco" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nco, resolution 1.8&Aring;" />
 
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'''STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN'''<br />
 
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==Overview==
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==STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN==
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Structures of the protein-chromophore complex and the apoprotein form of, neocarzinostatin were determined at 1.8 angstrom resolution., Neocarzinostatin is composed of a labile chromophore with DNA-cleaving, activity and a stabilizing protein. The chromophore displays marked, nonlinearity of the triple bonds and is bound noncovalently in a pocket, formed by the two protein domains. The chromophore pi-face interacts with, the phenyl ring edges of Phe52 and Phe78. The amino sugar and carbonate, groups of the chromophore are solvent exposed, whereas the epoxide, acetylene groups, and carbon C-12, the site of nucleophilic thiol addition, during chromophore activation, are unexposed. The position of the amino, group of the chromophore carbohydrate relative to C-12 supports the idea, that the amino group plays a role in thiol activation.
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<StructureSection load='1nco' size='340' side='right'caption='[[1nco]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nco]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NCO FirstGlance]. <br>
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1NCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with CHR and MRD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NCO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHR:NEOCARZINOSTATIN-CHROMOPHORE'>CHR</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nco OCA], [https://pdbe.org/1nco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nco RCSB], [https://www.ebi.ac.uk/pdbsum/1nco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nco ProSAT]</span></td></tr>
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Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex., Kim KH, Kwon BM, Myers AG, Rees DC, Science. 1993 Nov 12;262(5136):1042-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8235619 8235619]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/NCZS_STRCZ NCZS_STRCZ] NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nc/1nco_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nco ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces carzinostaticus]]
[[Category: Streptomyces carzinostaticus]]
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[[Category: Kim, K.H.]]
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[[Category: Kim K-H]]
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[[Category: Kwon, B.M.]]
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[[Category: Kwon B-M]]
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[[Category: Myers, A.G.]]
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[[Category: Myers AG]]
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[[Category: Rees, D.C.]]
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[[Category: Rees DC]]
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[[Category: CHR]]
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[[Category: MRD]]
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[[Category: antibacterial and antitumor protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:08:26 2007''
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Current revision

STRUCTURE OF THE ANTITUMOR PROTEIN-CHROMOPHORE COMPLEX NEOCARZINOSTATIN

PDB ID 1nco

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