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1nhl

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[[Image:1nhl.jpg|left|200px]]<br /><applet load="1nhl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nhl, resolution 2.3&Aring;" />
 
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'''SNAP-23N Structure'''<br />
 
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==Overview==
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==SNAP-23N Structure==
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SNARE proteins mediate intracellular membrane fusion by forming a, coiled-coil complex to merge opposing membranes. A "fusion-active", neuronal SNARE complex is a parallel four-helix bundle containing two, coiled-coil domains from SNAP-25 and one coiled-coil domain each from, syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can, also form from these SNAREs. We studied the N-terminal coiled-coil domain, of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its, interaction with other coiled-coil domains. SNAP-23N can assemble, spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and, VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N, crystallizes as a coiled-coil homotetrameric complex. The four helices, have a parallel orientation and are symmetrical about the long axis. The, complex is stabilized through the interaction of conserved hydrophobic, residues comprising the a and d positions of the coiled-coil heptad, repeats. In addition, a central, highly conserved glutamine residue, (Gln-48) is buried within the interface by hydrogen bonding between, glutamine side chains derived from adjacent subunits and to solvent, molecules. A comparison of the SNAP-23N structure to other SNARE complex, structures reveals how a simple coiled-coil motif can form diverse SNARE, complexes.
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<StructureSection load='1nhl' size='340' side='right'caption='[[1nhl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nhl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NHL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhl OCA], [https://pdbe.org/1nhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nhl RCSB], [https://www.ebi.ac.uk/pdbsum/1nhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nhl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNP23_HUMAN SNP23_HUMAN] Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nhl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nhl ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NHL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA].
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*[[Synaptosomal-associated protein|Synaptosomal-associated protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12556468 12556468]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Eck, M.J.]]
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[[Category: Eck MJ]]
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[[Category: Freedman, S.J.]]
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[[Category: Freedman SJ]]
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[[Category: Song, H.K.]]
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[[Category: Song HK]]
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[[Category: Xu, Y.]]
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[[Category: Xu Y]]
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[[Category: coiled-coil]]
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[[Category: snare]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:29:01 2008''
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Current revision

SNAP-23N Structure

PDB ID 1nhl

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