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1nio

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[[Image:1nio.gif|left|200px]]
 
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{{Structure
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==Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution==
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|PDB= 1nio |SIZE=350|CAPTION= <scene name='initialview01'>1nio</scene>, resolution 2.00&Aring;
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<StructureSection load='1nio' size='340' side='right'caption='[[1nio]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[1nio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NIO FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nio OCA], [https://pdbe.org/1nio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nio RCSB], [https://www.ebi.ac.uk/pdbsum/1nio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nio ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution'''
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== Function ==
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[https://www.uniprot.org/uniprot/RIPB_LUFAE RIPB_LUFAE]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The crystal structure of beta-luffin at 2.0 A resolution was solved by the molecular-replacement method using polyalanyl trichosanthin as the search model. The structure was refined with CNS1.1, giving R(work) = 0.162 and R(free) = 0.204. The r.m.s.d.s of the bond lengths and bond angles are 0.008 A and 1.3 degrees, respectively. The overall structure is similar to those of other type I RIPs. Three N-acetylglucosamine (Nag) molecules are linked to residues Asn2, Asn78 and Asn85 of the protein.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ni/1nio_consurf.spt"</scriptWhenChecked>
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1NIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876337 12876337]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nio ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Luffa aegyptiaca]]
[[Category: Luffa aegyptiaca]]
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[[Category: Single protein]]
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[[Category: Wu S]]
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[[Category: rRNA N-glycosylase]]
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[[Category: Wu, S.]]
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[[Category: NAG]]
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[[Category: beta-luffin]]
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[[Category: crystal structure]]
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[[Category: ribosome inactivating protein(rip)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:28 2008''
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Current revision

Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution

PDB ID 1nio

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