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1nlt

From Proteopedia

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(New page: 200px<br /><applet load="1nlt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nlt, resolution 2.70&Aring;" /> '''The crystal structur...)
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[[Image:1nlt.jpg|left|200px]]<br /><applet load="1nlt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nlt, resolution 2.70&Aring;" />
 
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'''The crystal structure of Hsp40 Ydj1'''<br />
 
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==Overview==
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==The crystal structure of Hsp40 Ydj1==
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The mechanisms by which Hsp40 functions as a molecular chaperone to, recognize and bind non-native polypeptides is not understood. We have, identified a peptide substrate for Ydj1, a member of the type I Hsp40 from, yeast. The structure of the Ydj1 peptide binding fragment and its peptide, substrate complex was determined to 2.7 A resolution. The complex, structure reveals that Ydj1 peptide binding fragment forms an L-shaped, molecule constituted by three domains. The domain I exhibits a similar, protein folds as domain III while the domain II contains two Zinc finger, motifs. The peptide substrate binds Ydj1 by forming an extra beta strand, with domain I of Ydj1. The Leucine residue in the middle of the peptide, substrate GWLYEIS inserts its side chain into a hydrophobic pocket formed, on the molecular surface of Ydj1 domain I. The Zinc finger motifs located, in the Ydj1 domain II are not in the vicinity of peptide substrate binding, site.
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<StructureSection load='1nlt' size='340' side='right'caption='[[1nlt]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nlt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NLT FirstGlance]. <br>
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1NLT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NLT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlt OCA], [https://pdbe.org/1nlt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nlt RCSB], [https://www.ebi.ac.uk/pdbsum/1nlt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nlt ProSAT]</span></td></tr>
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The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate., Li J, Qian X, Sha B, Structure. 2003 Dec;11(12):1475-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14656432 14656432]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MAS5_YEAST MAS5_YEAST] Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.<ref>PMID:11689685</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nl/1nlt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nlt ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Li J]]
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[[Category: Li, J.]]
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[[Category: Sha B]]
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[[Category: Sha, B.]]
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[[Category: ZN]]
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[[Category: beta-strands]]
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[[Category: chaperone]]
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[[Category: heat shock]]
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[[Category: mitochondrion]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:22:32 2007''
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Current revision

The crystal structure of Hsp40 Ydj1

PDB ID 1nlt

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