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1nlu

From Proteopedia

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(New page: 200px<br /><applet load="1nlu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nlu, resolution 1.30&Aring;" /> '''Pseudomonas sedolisi...)
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[[Image:1nlu.jpg|left|200px]]<br /><applet load="1nlu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nlu, resolution 1.30&Aring;" />
 
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'''Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin'''<br />
 
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==Overview==
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==Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin==
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High-resolution crystallographic analysis of a complex of the, serine-carboxyl proteinase sedolisin with pseudo-iodotyrostatin revealed, two molecules of this inhibitor bound in the active site of the enzyme, marking subsites from S3 to S3('). The mode of binding represents two, products of the proteolytic reaction. Substrate specificity of sedolisin, was investigated using peptide libraries and a new peptide substrate for, sedolisin, MCA-Lys-Pro-Pro-Leu-Glu#Tyr-Arg-Leu-Gly-Lys(DNP)-Gly, was, synthesized based on the results of the enzymatic and crystallographic, studies and was shown to be efficiently cleaved by the enzyme. The kinetic, parameters for the substrate, measured by the increase in fluorescence, upon relief of quenching, were: k(cat)=73+/-5 s(-1), K(m)=0.12+/-0.011, microM, and k(cat)/K(m)=608+/-85 s(-1)microM(-1).
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<StructureSection load='1nlu' size='340' side='right'caption='[[1nlu]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nlu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NLU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=PHI:IODO-PHENYLALANINE'>PHI</scene>, <scene name='pdbligand=TYB:TYROSINAL'>TYB</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nlu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nlu OCA], [https://pdbe.org/1nlu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nlu RCSB], [https://www.ebi.ac.uk/pdbsum/1nlu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nlu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PICP_PSESR PICP_PSESR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nl/1nlu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nlu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NLU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudomonalisin Pseudomonalisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.100 3.4.21.100] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NLU OCA].
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*[[Pepsin|Pepsin]]
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*[[Proteinase 3D structures|Proteinase 3D structures]]
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==Reference==
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__TOC__
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Two inhibitor molecules bound in the active site of Pseudomonas sedolisin: a model for the bi-product complex following cleavage of a peptide substrate., Wlodawer A, Li M, Gustchina A, Oyama H, Oda K, Beyer BB, Clemente J, Dunn BM, Biochem Biophys Res Commun. 2004 Feb 6;314(2):638-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14733955 14733955]
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</StructureSection>
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[[Category: Pseudomonalisin]]
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[[Category: Large Structures]]
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[[Category: Pseudomonas sp.]]
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[[Category: Pseudomonas sp]]
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[[Category: Single protein]]
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[[Category: Dauter Z]]
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[[Category: Dauter, Z.]]
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[[Category: Dunn BM]]
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[[Category: Dunn, B.M.]]
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[[Category: Glodfarb NE]]
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[[Category: Glodfarb, N.E.]]
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[[Category: Gustchina A]]
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[[Category: Gustchina, A.]]
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[[Category: Li M]]
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[[Category: Li, M.]]
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[[Category: Oda K]]
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[[Category: Oda, K.]]
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[[Category: Oyama H]]
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[[Category: Oyama, H.]]
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[[Category: Uchida K]]
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[[Category: Uchida, K.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: CA]]
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[[Category: pscp]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:22:37 2007''
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Current revision

Pseudomonas sedolisin (serine-carboxyl proteinase) complexed with two molecules of pseudo-iodotyrostatin

PDB ID 1nlu

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