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1nz0

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[[Image:1nz0.gif|left|200px]]<br /><applet load="1nz0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nz0, resolution 1.20&Aring;" />
 
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'''RNASE P PROTEIN FROM THERMOTOGA MARITIMA'''<br />
 
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==Overview==
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==RNASE P PROTEIN FROM THERMOTOGA MARITIMA==
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The structure of RNase P protein from the hyperthermophilic bacterium Thermotoga maritima was determined at 1.2-A resolution by using x-ray crystallography. This protein structure is from an ancestral-type RNase P and bears remarkable similarity to the recently determined structures of RNase P proteins from bacteria that have the distinct, Bacillus type of RNase P. These two types of protein span the extent of bacterial RNase P diversity, so the results generalize the structure of the bacterial RNase P protein. The broad phylogenetic conservation of structure and distribution of potential RNA-binding elements in the RNase P proteins indicate that all of these homologous proteins bind to their cognate RNAs primarily by interaction with the phylogenetically conserved core of the RNA. The protein is found to dimerize through an extensive, well-ordered interface. This dimerization may reflect a mechanism of thermal stability of the protein before assembly with the RNA moiety of the holoenzyme.
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<StructureSection load='1nz0' size='340' side='right'caption='[[1nz0]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nz0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZ0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nz0 OCA], [https://pdbe.org/1nz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1nz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nz0 ProSAT], [https://www.topsan.org/Proteins/BSGC/1nz0 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNPA_THEMA RNPA_THEMA] RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/1nz0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nz0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_P Ribonuclease P], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.5 3.1.26.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZ0 OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution structure of RNase P protein from Thermotoga maritima., Kazantsev AV, Krivenko AA, Harrington DJ, Carter RJ, Holbrook SR, Adams PD, Pace NR, Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7497-502. Epub 2003 Jun 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12799461 12799461]
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[[Category: Large Structures]]
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[[Category: Ribonuclease P]]
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[[Category: Single protein]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Adams, P D.]]
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[[Category: Adams PD]]
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[[Category: BSGC, Berkeley Structural Genomics Center.]]
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[[Category: Carter RJ]]
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[[Category: Carter, R J.]]
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[[Category: Harrington DJ]]
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[[Category: Harrington, D J.]]
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[[Category: Holbrook SR]]
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[[Category: Holbrook, S R.]]
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[[Category: Kazantsev AV]]
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[[Category: Kazantsev, A V.]]
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[[Category: Krivenko AA]]
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[[Category: Krivenko, A A.]]
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[[Category: Pace NR]]
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[[Category: Pace, N R.]]
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[[Category: SO4]]
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[[Category: alfa-beta sandwich]]
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[[Category: berkeley structural genomics center]]
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[[Category: bsgc structure funded by nih]]
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[[Category: dimer]]
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[[Category: endonuclease]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: rnase]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:36 2008''
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Current revision

RNASE P PROTEIN FROM THERMOTOGA MARITIMA

PDB ID 1nz0

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