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1nz6

From Proteopedia

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[[Image:1nz6.jpg|left|200px]]<br /><applet load="1nz6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nz6, resolution 2.5&Aring;" />
 
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'''Crystal Structure of Auxilin J-Domain'''<br />
 
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==Overview==
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==Crystal Structure of Auxilin J-Domain==
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J-domains are widespread protein interaction modules involved in recruiting and stimulating the activity of Hsp70 family chaperones. We have determined the crystal structure of the J-domain of auxilin, a protein which is involved in uncoating clathrin-coated vesicles. Comparison to the known structures of J-domains from four other proteins reveals that the auxilin J-domain is the most divergent of all J-domain structures described to date. In addition to the canonical J-domain features described previously, the auxilin J-domain contains an extra N-terminal helix and a long loop inserted between helices I and II. The latter loop extends the positively charged surface which forms the Hsc70 binding site, and is shown by directed mutagenesis and surface plasmon resonance to contain side chains important for binding to Hsc70.
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<StructureSection load='1nz6' size='340' side='right'caption='[[1nz6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nz6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZ6 FirstGlance]. <br>
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1NZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZ6 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nz6 OCA], [https://pdbe.org/1nz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nz6 RCSB], [https://www.ebi.ac.uk/pdbsum/1nz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nz6 ProSAT]</span></td></tr>
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Structure-function analysis of the auxilin J-domain reveals an extended Hsc70 interaction interface., Jiang J, Taylor AB, Prasad K, Ishikawa-Brush Y, Hart PJ, Lafer EM, Sousa R, Biochemistry. 2003 May 20;42(19):5748-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12741832 12741832]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AUXI_BOVIN AUXI_BOVIN] Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles.<ref>PMID:15502813</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/1nz6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nz6 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hart, P J.]]
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[[Category: Hart PJ]]
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[[Category: Ishikawa-Brush, Y.]]
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[[Category: Ishikawa-Brush Y]]
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[[Category: Jiang, J.]]
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[[Category: Jiang J]]
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[[Category: Lafer, E M.]]
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[[Category: Lafer EM]]
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[[Category: Prasad, K.]]
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[[Category: Prasad K]]
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[[Category: Sousa, R.]]
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[[Category: Sousa R]]
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[[Category: Taylor, A B.]]
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[[Category: Taylor AB]]
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[[Category: alpha helix]]
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[[Category: anti-parallel helix hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:38 2008''
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Current revision

Crystal Structure of Auxilin J-Domain

PDB ID 1nz6

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