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1o95

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[[Image:1o95.gif|left|200px]]<br />
 
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<applet load="1o95" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1o95, resolution 3.70&Aring;" />
 
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'''TERNARY COMPLEX BETWEEN TRIMETHYLAMINE DEHYDROGENASE AND ELECTRON TRANSFERRING FLAVOPROTEIN'''<br />
 
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==Overview==
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==Ternary complex between trimethylamine dehydrogenase and electron transferring flavoprotein==
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Here we report the crystal structures of a ternary electron transfer, complex showing extensive motion at the protein interface. This, physiological complex comprises the iron-sulfur flavoprotein, trimethylamine dehydrogenase and electron transferring flavoprotein (ETF), from Methylophilus methylotrophus. In addition, we report the crystal, structure of free ETF. In the complex, electron density for the FAD domain, of ETF is absent, indicating high mobility. Positions for the FAD domain, are revealed by molecular dynamics simulation, consistent with crystal, structures and kinetic data. A dual interaction of ETF with trimethylamine, dehydrogenase provides for dynamical motion at the protein interface: one, site acts as an anchor, thereby allowing the other site to sample a large, range of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12567183 (full description)]]
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<StructureSection load='1o95' size='340' side='right'caption='[[1o95]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1o95]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O95 FirstGlance]. <br>
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1O95 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]] with FMN, ADP, SF4 and AMP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_1.5.8.2 Transferred entry: 1.5.8.2]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.7 1.5.99.7]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O95 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o95 OCA], [https://pdbe.org/1o95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o95 RCSB], [https://www.ebi.ac.uk/pdbsum/1o95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o95 ProSAT]</span></td></tr>
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Extensive conformational sampling in a ternary electron transfer complex., Leys D, Basran J, Talfournier F, Sutcliffe MJ, Scrutton NS, Nat Struct Biol. 2003 Mar;10(3):219-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12567183 12567183]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHTM_METME DHTM_METME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/1o95_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o95 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methylophilus methylotrophus]]
[[Category: Methylophilus methylotrophus]]
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[[Category: Protein complex]]
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[[Category: Basran J]]
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[[Category: Transferred entry: 1.5.8.2]]
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[[Category: Leys D]]
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[[Category: Basran, J.]]
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[[Category: Scrutton NS]]
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[[Category: Leys, D.]]
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[[Category: Sutcliffe MJ]]
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[[Category: Scrutton, N.S.]]
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[[Category: Talfournier F]]
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[[Category: Sutcliffe, M.J.]]
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[[Category: Talfournier, F.]]
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[[Category: ADP]]
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[[Category: AMP]]
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[[Category: FMN]]
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[[Category: SF4]]
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[[Category: dehydrogenase]]
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[[Category: electron transfer]]
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[[Category: electron transport]]
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[[Category: flavoprotein]]
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[[Category: fmn]]
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[[Category: iron-sulfur]]
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[[Category: oxido-reductase]]
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[[Category: protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:02:20 2007''
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Current revision

Ternary complex between trimethylamine dehydrogenase and electron transferring flavoprotein

PDB ID 1o95

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