This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1o95

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:56, 10 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1o95.jpg|left|200px]]
 
-
{{Structure
+
==Ternary complex between trimethylamine dehydrogenase and electron transferring flavoprotein==
-
|PDB= 1o95 |SIZE=350|CAPTION= <scene name='initialview01'>1o95</scene>, resolution 3.70&Aring;
+
<StructureSection load='1o95' size='340' side='right'caption='[[1o95]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
-
|SITE= <scene name='pdbsite=AC1:Fs4+Binding+Site+For+Chain+B'>AC1</scene>
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene>
+
<table><tr><td colspan='2'>[[1o95]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O95 FirstGlance]. <br>
-
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_1.5.8.2 Transferred entry: 1.5.8.2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.7 1.5.99.7]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o95 OCA], [https://pdbe.org/1o95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o95 RCSB], [https://www.ebi.ac.uk/pdbsum/1o95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o95 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''TERNARY COMPLEX BETWEEN TRIMETHYLAMINE DEHYDROGENASE AND ELECTRON TRANSFERRING FLAVOPROTEIN'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/DHTM_METME DHTM_METME]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Here we report the crystal structures of a ternary electron transfer complex showing extensive motion at the protein interface. This physiological complex comprises the iron-sulfur flavoprotein trimethylamine dehydrogenase and electron transferring flavoprotein (ETF) from Methylophilus methylotrophus. In addition, we report the crystal structure of free ETF. In the complex, electron density for the FAD domain of ETF is absent, indicating high mobility. Positions for the FAD domain are revealed by molecular dynamics simulation, consistent with crystal structures and kinetic data. A dual interaction of ETF with trimethylamine dehydrogenase provides for dynamical motion at the protein interface: one site acts as an anchor, thereby allowing the other site to sample a large range of interactions, some compatible with rapid electron transfer. This study establishes the role of conformational sampling in multi-domain redox systems, providing insight into electron transfer between ETFs and structurally distinct redox partners.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/1o95_consurf.spt"</scriptWhenChecked>
-
1O95 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O95 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Extensive conformational sampling in a ternary electron transfer complex., Leys D, Basran J, Talfournier F, Sutcliffe MJ, Scrutton NS, Nat Struct Biol. 2003 Mar;10(3):219-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12567183 12567183]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o95 ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Methylophilus methylotrophus]]
[[Category: Methylophilus methylotrophus]]
-
[[Category: Protein complex]]
+
[[Category: Basran J]]
-
[[Category: Transferred entry: 1 5.8 2]]
+
[[Category: Leys D]]
-
[[Category: Basran, J.]]
+
[[Category: Scrutton NS]]
-
[[Category: Leys, D.]]
+
[[Category: Sutcliffe MJ]]
-
[[Category: Scrutton, N S.]]
+
[[Category: Talfournier F]]
-
[[Category: Sutcliffe, M J.]]
+
-
[[Category: Talfournier, F.]]
+
-
[[Category: ADP]]
+
-
[[Category: AMP]]
+
-
[[Category: FMN]]
+
-
[[Category: SF4]]
+
-
[[Category: dehydrogenase]]
+
-
[[Category: electron transfer]]
+
-
[[Category: electron transport]]
+
-
[[Category: flavoprotein]]
+
-
[[Category: fmn]]
+
-
[[Category: iron-sulfur]]
+
-
[[Category: oxido-reductase]]
+
-
[[Category: protein complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:01:37 2008''
+

Current revision

Ternary complex between trimethylamine dehydrogenase and electron transferring flavoprotein

PDB ID 1o95

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1o95"
Personal tools