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1o9b

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[[Image:1o9b.gif|left|200px]]<br />
 
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<applet load="1o9b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1o9b, resolution 2.50&Aring;" />
 
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'''QUINATE/SHIKIMATE DEHYDROGENASE YDIB COMPLEXED WITH NADH'''<br />
 
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==Overview==
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==QUINATE/SHIKIMATE DEHYDROGENASE YDIB COMPLEXED WITH NADH==
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Shikimate dehydrogenase catalyzes the fourth step of the shikimate, pathway, the essential route for the biosynthesis of aromatic compounds in, plants and microorganisms. Absent in metazoans, this pathway is an, attractive target for nontoxic herbicides and drugs. Escherichia coli, expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and, a putative enzyme YdiB. Here we characterize YdiB as a dual specificity, quinate/shikimate dehydrogenase that utilizes either NAD or NADP as a, cofactor. Structures of AroE and YdiB with bound cofactors were determined, at 1.5 and 2.5 A resolution, respectively. Both enzymes display a similar, architecture with two alpha/beta domains separated by a wide cleft., Comparison of their dinucleotide-binding domains reveals the molecular, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12637497 (full description)]]
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<StructureSection load='1o9b' size='340' side='right'caption='[[1o9b]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1o9b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O9B FirstGlance]. <br>
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1O9B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PO4 and NAD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25]]. Structure known Active Site: NAA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O9B OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o9b OCA], [https://pdbe.org/1o9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o9b RCSB], [https://www.ebi.ac.uk/pdbsum/1o9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o9b ProSAT], [https://www.topsan.org/Proteins/BSGI/1o9b TOPSAN]</span></td></tr>
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Structures of shikimate dehydrogenase AroE and its Paralog YdiB. A common structural framework for different activities., Michel G, Roszak AW, Sauve V, Maclean J, Matte A, Coggins JR, Cygler M, Lapthorn AJ, J Biol Chem. 2003 May 23;278(21):19463-72. Epub 2003 Mar 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12637497 12637497]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YDIB_ECOLI YDIB_ECOLI] The physiological substrate is not known.<ref>PMID:12624088</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/1o9b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o9b ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Shikimate dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cygler M]]
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[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
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[[Category: Michel G]]
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[[Category: Cygler, M.]]
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[[Category: Michel, G.]]
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[[Category: NAD]]
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[[Category: PO4]]
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[[Category: bsgi]]
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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[[Category: nad]]
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[[Category: oxidoreductase]]
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[[Category: quinate]]
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[[Category: shikimate]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:47:07 2007''
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Current revision

QUINATE/SHIKIMATE DEHYDROGENASE YDIB COMPLEXED WITH NADH

PDB ID 1o9b

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