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1oay

From Proteopedia

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[[Image:1oay.jpg|left|200px]]
 
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{{Structure
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==Antibody multispecificity mediated by conformational diversity==
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|PDB= 1oay |SIZE=350|CAPTION= <scene name='initialview01'>1oay</scene>, resolution 2.66&Aring;
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<StructureSection load='1oay' size='340' side='right'caption='[[1oay]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Fur+Binding+Site+For+Chain+J'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FUR:ACENAPHTHENEQUINONE'>FUR</scene>
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<table><tr><td colspan='2'>[[1oay]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OAY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUR:FURAZOLIDONE'>FUR</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oay OCA], [https://pdbe.org/1oay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oay RCSB], [https://www.ebi.ac.uk/pdbsum/1oay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oay ProSAT]</span></td></tr>
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</table>
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'''ANTIBODY MULTISPECIFICITY MEDIATED BY CONFORMATIONAL DIVERSITY'''
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== Function ==
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[https://www.uniprot.org/uniprot/LV1B_MOUSE LV1B_MOUSE]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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A single antibody was shown to adopt different binding-site conformations and thereby bind unrelated antigens. Analysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between different preexisting isomers, one of which possessed a promiscuous, low-affinity binding site for aromatic ligands, including the immunizing hapten. A subsequent induced-fit isomerization led to high-affinity complexes with a deep and narrow binding site. A protein antigen identified by repertoire selection made use of an unrelated antibody isomer with a wide, shallow binding site. Conformational diversity, whereby one sequence adopts multiple structures and multiple functions, can increase the effective size of the antibody repertoire but may also lead to autoimmunity and allergy.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oay_consurf.spt"</scriptWhenChecked>
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1OAY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OAY OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Antibody multispecificity mediated by conformational diversity., James LC, Roversi P, Tawfik DS, Science. 2003 Feb 28;299(5611):1362-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12610298 12610298]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oay ConSurf].
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[[Category: Protein complex]]
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<div style="clear:both"></div>
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[[Category: Rattus rattus]]
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__TOC__
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[[Category: James, L C.]]
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</StructureSection>
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[[Category: Roversi, P.]]
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[[Category: Large Structures]]
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[[Category: Tawfik, D.]]
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[[Category: Mus musculus]]
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[[Category: FUR]]
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[[Category: James LC]]
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[[Category: allergy]]
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[[Category: Roversi P]]
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[[Category: antibody]]
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[[Category: Tawfik D]]
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[[Category: conformational diversity]]
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[[Category: ige]]
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[[Category: multispecificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:08:17 2008''
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Current revision

Antibody multispecificity mediated by conformational diversity

PDB ID 1oay

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