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1ohm

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[[Image:1ohm.jpg|left|200px]]
 
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==Sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge.==
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The line below this paragraph, containing "STRUCTURE_1ohm", creates the "Structure Box" on the page.
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<StructureSection load='1ohm' size='340' side='right'caption='[[1ohm]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ohm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Latilactobacillus_sakei Latilactobacillus sakei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohm OCA], [https://pdbe.org/1ohm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohm RCSB], [https://www.ebi.ac.uk/pdbsum/1ohm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohm ProSAT]</span></td></tr>
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{{STRUCTURE_1ohm| PDB=1ohm | SCENE= }}
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</table>
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== Function ==
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'''SAKACIN P VARIANT THAT IS STRUCTURALLY STABILIZED BY AN INSERTED C-TERMINAL DISULFIDE BRIDGE.'''
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[https://www.uniprot.org/uniprot/SAKP_LATSK SAKP_LATSK] Bactericidal activity; inhibits closely related Lactobacilli, Listeria monocytogenes and ivanovvi, Enterococcus faecalis, Carnobacterium sp and Brocothrix thermosphacta.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The three-dimensional structures in dodecylphosphocholine (DPC) micelles and in trifluoroethanol (TFE) of the pediocin-like antimicrobial peptide sakacin P and an engineered variant of sakacin P (termed sakP[N24C+44C]) have been determined by use of nuclear magnetic resonance spectroscopy. SakP[N24C+44C] has an inserted non-native activity- and structure-stabilizing C-terminal disulfide bridge that ties the C-terminus to the middle part of the peptide. In the presence of DPC, the cationic N-terminal region (residues 1-17) of both peptides has an S-shaped conformation that is reminiscent of a three-stranded antiparallel beta-sheet and that is more pronounced when the peptide was dissolved in TFE instead of DPC. The four positively charged residues located in the N-terminal part are found pointing to the same direction. For both peptides, the N-terminal region is followed by a well-defined central amphiphilic alpha-helix (residues 18-33), and this in turn is followed by the C-terminal tail (residues 34-43 for sakacin P and 34-44 for sakP[N24C+44C]) that lacks any apparent common secondary structural motif. In the presence of DPC, the C-terminal tails in both peptides fold back onto the central alpha-helix, thereby creating a hairpin-like structure in the C-terminal halves. The lack of long-range NOEs between the beta-sheet Nu-terminal region and the hairpin-like C-terminal half indicates that there is a flexible hinge between these regions. We discuss which implications such a structural arrangement has on the interaction with the target cell membrane.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohm_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1OHM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_sakei Lactobacillus sakei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHM OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohm ConSurf].
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Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge., Uteng M, Hauge HH, Markwick PR, Fimland G, Mantzilas D, Nissen-Meyer J, Muhle-Goll C, Biochemistry. 2003 Oct 7;42(39):11417-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14516192 14516192]
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<div style="clear:both"></div>
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[[Category: Lactobacillus sakei]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Fimland, G.]]
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[[Category: Large Structures]]
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[[Category: Hauge, H H.]]
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[[Category: Latilactobacillus sakei]]
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[[Category: Mantzilas, D.]]
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[[Category: Fimland G]]
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[[Category: Markwick, P.]]
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[[Category: Hauge HH]]
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[[Category: Muhle-Goll, C.]]
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[[Category: Mantzilas D]]
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[[Category: Nissen-Meyer, J.]]
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[[Category: Markwick PR]]
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[[Category: Uteng, M.]]
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[[Category: Muhle-Goll C]]
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[[Category: Antimicrobial peptide]]
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[[Category: Nissen-Meyer J]]
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[[Category: Bacteriocin]]
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[[Category: Uteng M]]
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[[Category: Pediocin-like bacteriocin]]
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[[Category: Sakacin antibiotic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:51:30 2008''
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Current revision

Sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge.

PDB ID 1ohm

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