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1ohw

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(New page: 200px<br /> <applet load="1ohw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ohw, resolution 2.30&Aring;" /> '''4-AMINOBUTYRATE-AMI...)
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[[Image:1ohw.gif|left|200px]]<br />
 
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<applet load="1ohw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ohw, resolution 2.30&Aring;" />
 
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'''4-AMINOBUTYRATE-AMINOTRANSFERASE INACTIVATED BY GAMMA-VINYL GABA'''<br />
 
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==Overview==
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==4-AMINOBUTYRATE-AMINOTRANSFERASE inactivated by gamma-vinyl GABA==
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Gamma-aminobutyric acid aminotransferase (GABA-AT) is a pyridoxal, 5'-phosphate-dependent enzyme responsible for the degradation of the, inhibitory neurotransmitter GABA. GABA-AT is a validated target for, antiepilepsy drugs because its selective inhibition raises GABA, concentrations in brain. The antiepilepsy drug, gamma-vinyl-GABA, (vigabatrin) has been investigated in the past by various biochemical, methods and resulted in several proposals for its mechanisms of, inactivation. In this study we solved and compared the crystal structures, of pig liver GABA-AT in its native form (to 2.3-A resolution) and in, complex with vigabatrin as well as with the close analogue, gamma-ethynyl-GABA (to 2.3 and 2.8 A, respectively). Both inactivators, form a covalent ternary adduct with the active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14534310 (full description)]]
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<StructureSection load='1ohw' size='340' side='right'caption='[[1ohw]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ohw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHW FirstGlance]. <br>
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1OHW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with FE, PLP and S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.19 2.6.1.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OHW OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=VIG:4-AMINO+HEXANOIC+ACID'>VIG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohw OCA], [https://pdbe.org/1ohw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohw RCSB], [https://www.ebi.ac.uk/pdbsum/1ohw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohw ProSAT]</span></td></tr>
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Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin., Storici P, De Biase D, Bossa F, Bruno S, Mozzarelli A, Peneff C, Silverman RB, Schirmer T, J Biol Chem. 2004 Jan 2;279(1):363-73. Epub 2003 Oct 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14534310 14534310]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/GABT_PIG GABT_PIG] Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohw ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Schirmer, T.]]
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[[Category: Schirmer T]]
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[[Category: Storici, P.]]
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[[Category: Storici P]]
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[[Category: FE]]
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[[Category: PLP]]
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[[Category: S]]
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[[Category: 4-aminobutyric acid]]
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[[Category: aminotransferase]]
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[[Category: antiepileptic drug target]]
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[[Category: mitochondrion]]
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[[Category: neurotransmitter degradation]]
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[[Category: plp-dependent enzyme]]
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[[Category: transferase]]
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[[Category: transit peptide]]
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[[Category: vigabatrin pyridoxal phosphate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:35:20 2007''
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Current revision

4-AMINOBUTYRATE-AMINOTRANSFERASE inactivated by gamma-vinyl GABA

PDB ID 1ohw

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