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1okd
From Proteopedia
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| - | ==NMR- | + | |
| - | <StructureSection load='1okd' size='340' side='right' caption='[[1okd | + | ==NMR-structure of tryparedoxin 1== |
| + | <StructureSection load='1okd' size='340' side='right'caption='[[1okd]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1okd]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1okd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OKD FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1okd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1okd OCA], [https://pdbe.org/1okd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1okd RCSB], [https://www.ebi.ac.uk/pdbsum/1okd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1okd ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O96438_CRIFA O96438_CRIFA] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/1okd_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/1okd_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 16: | Line 19: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1okd ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1okd ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Tryparedoxins (TXNs) are trypanothione-dependent peroxiredoxin oxidoreductases involved in hydroperoxide detoxification that have been shown to determine virulence in trypanosomatids. The structure of (15)N,(13)C-doubly-labeled, C-terminally-His-tagged tryparedoxin 1 from Crithidia fasciculata (Cf TXN1) was elucidated by three-dimensional NMR spectroscopy. Global folding was found to be similar to the crystal structure, but regions near the active site, especially the onset of helix alpha1 with the redox-active Cys 43 and helix alpha2 relevant to substrate binding, were less well defined in solution. The redox-inactive inhibitory substrate analogue N(1),N(8)-bis(ophthalmyl)spermidine was used to study the substrate/TXN interaction by two-dimensional (1)H,(15)N NMR spectroscopy. The NMR data complemented by molecular modeling revealed several alternative modes of ligand binding. The results confirm and extend the concept of TXN action and specificity derived from X-ray analysis and site-directed mutagenesis and thus improve the rational basis for inhibitor design. | ||
| - | |||
| - | NMR studies of the interaction of tryparedoxin with redox-inactive substrate homologues.,Krumme D, Budde H, Hecht HJ, Menge U, Ohlenschlager O, Ross A, Wissing J, Wray V, Flohe L Biochemistry. 2003 Dec 23;42(50):14720-8. PMID:14674746<ref>PMID:14674746</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1okd" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Crithidia fasciculata]] |
| - | [[Category: Budde | + | [[Category: Large Structures]] |
| - | [[Category: Flohe | + | [[Category: Budde H]] |
| - | [[Category: Hecht | + | [[Category: Flohe L]] |
| - | [[Category: Krumme | + | [[Category: Hecht H-J]] |
| - | [[Category: Menge | + | [[Category: Krumme D]] |
| - | [[Category: Ohlenschlager | + | [[Category: Menge U]] |
| - | [[Category: Ross | + | [[Category: Ohlenschlager O]] |
| - | [[Category: Wissing | + | [[Category: Ross A]] |
| - | [[Category: Wray | + | [[Category: Wissing J]] |
| - | + | [[Category: Wray V]] | |
| - | + | ||
| - | + | ||
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Current revision
NMR-structure of tryparedoxin 1
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Categories: Crithidia fasciculata | Large Structures | Budde H | Flohe L | Hecht H-J | Krumme D | Menge U | Ohlenschlager O | Ross A | Wissing J | Wray V
