1okh

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[[Image:1okh.gif|left|200px]]<br />
 
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<applet load="1okh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1okh, resolution 1.75&Aring;" />
 
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'''VISCOTOXIN A3 FROM VISCUM ALBUM L.'''<br />
 
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==Overview==
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==Viscotoxin A3 from Viscum album L.==
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The crystal structure of viscotoxin A3 (VT A3) extracted from European, mistletoe (Viscum album L.) has been solved using the anomalous, diffraction of the native S atoms measured in-house with Cu Kalpha, radiation to a resolution of 2.2 A and truncated to 2.5 A. A 1.75 A, resolution synchrotron data set was used for phase expansion and, refinement. An innovation in the dual-space substructure-solution program, SHELXD enabled the individual S atoms of the disulfide bonds to be located, using the Cu Kalpha data; this resulted in a marked improvement in the, phasing compared with the use of super-S atoms. The VT A3 monomer consists, of 46 amino acids with three disulfide bridges and has an overall fold, resembling the canonical architecture of the alpha- and beta-thionins, a, capital letter L. The asymmetric unit consists of two monomers related by, a local twofold axis and held together by hydrophobic interactions between, the monomer units. One phosphate anion (confirmed by 31P-NMR and MS) is, associated with each monomer.
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<StructureSection load='1okh' size='340' side='right'caption='[[1okh]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1okh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OKH FirstGlance]. <br>
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1OKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album] with PO4 and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OKH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1okh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1okh OCA], [https://pdbe.org/1okh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1okh RCSB], [https://www.ebi.ac.uk/pdbsum/1okh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1okh ProSAT]</span></td></tr>
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Structure of viscotoxin A3: disulfide location from weak SAD data., Debreczeni JE, Girmann B, Zeeck A, Kratzner R, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2125-32. Epub 2003, Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14646070 14646070]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/THN3_VISAL THN3_VISAL] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/1okh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1okh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Viscum album]]
[[Category: Viscum album]]
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[[Category: Debreczeni, J.E.]]
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[[Category: Debreczeni JE]]
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[[Category: Girmann, B.]]
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[[Category: Girmann B]]
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[[Category: Sheldrick, G.M.]]
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[[Category: Sheldrick GM]]
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[[Category: Zeeck, A.]]
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[[Category: Zeeck A]]
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[[Category: PO4]]
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[[Category: SO4]]
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[[Category: plant defense]]
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[[Category: thionin]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:31:21 2007''
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Current revision

Viscotoxin A3 from Viscum album L.

PDB ID 1okh

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