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1oof

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(New page: 200px<br /><applet load="1oof" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oof, resolution 1.49&Aring;" /> '''Complex of Drosophil...)
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[[Image:1oof.jpg|left|200px]]<br /><applet load="1oof" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oof, resolution 1.49&Aring;" />
 
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'''Complex of Drosophila odorant binding protein LUSH with ethanol'''<br />
 
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==Overview==
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==Complex of Drosophila odorant binding protein LUSH with ethanol==
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We have solved the high-resolution crystal structures of the Drosophila, melanogaster alcohol-binding protein LUSH in complex with a series of, short-chain n-alcohols. LUSH is the first known nonenzyme protein with a, defined in vivo alcohol-binding function. The structure of LUSH reveals a, set of molecular interactions that define a specific alcohol-binding site., A group of amino acids, Thr57, Ser52 and Thr48, form a network of, concerted hydrogen bonds between the protein and the alcohol that provides, a structural motif to increase alcohol-binding affinity at this site. This, motif seems to be conserved in a number of mammalian ligand-gated ion, channels that are directly implicated in the pharmacological effects of, alcohol. Further, these sequences are found in regions of ion channels, that are known to confer alcohol sensitivity. We suggest that the, alcohol-binding site in LUSH represents a general model for, alcohol-binding sites in proteins.
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<StructureSection load='1oof' size='340' side='right'caption='[[1oof]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1oof]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OOF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oof OCA], [https://pdbe.org/1oof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oof RCSB], [https://www.ebi.ac.uk/pdbsum/1oof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OB76A_DROME OB76A_DROME] Odorant-binding protein required for olfactory behavior and for activity of pheromone-sensitive neurons. Binds to alcohols and mediates avoidance behavior to high concentrations of alcohols, the alcohol-binding possibly resulting in activation of receptors on T2B neurons, the activation of these receptors inhibiting these neurons. Acts in concert with Snmp and lush to capture cVA molecules on the surface of Or67d expressing olfactory dendrites and facilitate their transfer to the odorant-receptor Orco complex. Required for cVA response, probably by binding to VA. May act by serving as an adapter that bridges the presence of gaseous pheromone molecules, cVA, to activation of specific neuronal receptors expressed on T1 olfactory neurons, possibly via a specific conformational change induced by cVA that in turn activates T1 receptors. T1 neurons are excited by the pheromone VA, while T2 neurons are inhibited by alcohols. Also binds to phthalates.<ref>PMID:9755202</ref> <ref>PMID:11238251</ref> <ref>PMID:14759510</ref> <ref>PMID:15664171</ref> <ref>PMID:16928861</ref> <ref>PMID:17943085</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/1oof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oof ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OOF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ACT and EOH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OOF OCA].
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*[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster., Kruse SW, Zhao R, Smith DP, Jones DN, Nat Struct Biol. 2003 Sep;10(9):694-700. Epub 2003 Jul 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12881720 12881720]
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jones, D.N.M.]]
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[[Category: Jones DNM]]
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[[Category: Kruse, S.W.]]
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[[Category: Kruse SW]]
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[[Category: Smith, D.P.]]
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[[Category: Smith DP]]
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[[Category: Zhao, R.]]
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[[Category: Zhao R]]
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[[Category: ACT]]
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[[Category: EOH]]
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[[Category: alcohol]]
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[[Category: lush]]
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[[Category: odorant binding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:03:51 2007''
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Current revision

Complex of Drosophila odorant binding protein LUSH with ethanol

PDB ID 1oof

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