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1or0

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[[Image:1or0.jpg|left|200px]]
 
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==Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation==
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The line below this paragraph, containing "STRUCTURE_1or0", creates the "Structure Box" on the page.
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<StructureSection load='1or0' size='340' side='right'caption='[[1or0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1or0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._SY-77-1 Pseudomonas sp. SY-77-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OR0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1or0| PDB=1or0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1or0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1or0 OCA], [https://pdbe.org/1or0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1or0 RCSB], [https://www.ebi.ac.uk/pdbsum/1or0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1or0 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation'''
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== Function ==
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[https://www.uniprot.org/uniprot/G7AC_PSEU7 G7AC_PSEU7] Catalyzes the deacylation of 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl-7-aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA).<ref>PMID:2993240</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Glutaryl 7-aminocephalosporanic acid acylase (GCA, EC 3.5.1.11) is a member of N-terminal nucleophile (Ntn) hydrolases. The native enzyme is an (alpha beta)(2) heterotetramer originated from an enzymatically inactive precursor of a single polypeptide. The activation of precursor GCA consists of primary and secondary autoproteolytic cleavages, generating a terminal residue with both a nucleophile and a base and releasing a nine amino acid spacer peptide. We have determined the crystal structures of the recombinant selenomethionyl native and S170A mutant precursor from Pseudomonas sp. strain GK16. Precursor activation is likely triggered by conformational constraints within the spacer peptide, probably inducing a peptide flip. Autoproteolytic site solvent molecules, which have been trapped in a hydrophobic environment by the spacer peptide, may play a role as a general base for nucleophilic attack. The activation results in building up a catalytic triad composed of Ser170/His192/Glu624. However, the triad is not linked to the usual hydroxyl but the free alpha-amino group of the N-terminal serine residue of the native GCA. Mutagenesis and structural data support the notion that the stabilization of a transient hydroxazolidine ring during autoproteolysis would be critical during the N --&gt; O acyl shift. The autoproteolytic activation mechanism for GCA is described.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/1or0_consurf.spt"</scriptWhenChecked>
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1OR0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp._sy-77 Pseudomonas sp. sy-77]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OR0 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation., Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH, Biochemistry. 2003 Apr 15;42(14):4084-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12680762 12680762]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1or0 ConSurf].
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[[Category: Protein complex]]
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<div style="clear:both"></div>
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[[Category: Pseudomonas sp. sy-77]]
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== References ==
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[[Category: Dauter, Z.]]
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<references/>
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[[Category: Kim, J K.]]
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__TOC__
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[[Category: Kim, K H.]]
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</StructureSection>
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[[Category: Lee, Y S.]]
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[[Category: Large Structures]]
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[[Category: Park, S S.]]
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[[Category: Pseudomonas sp. SY-77-1]]
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[[Category: Rhee, S.]]
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[[Category: Dauter Z]]
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[[Category: Yang, I S.]]
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[[Category: Kim JK]]
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[[Category: Glutaryl 7-aminocephalosporanic acid]]
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[[Category: Kim KH]]
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[[Category: Glutaryl 7-aminocephalosporanic acid acylase]]
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[[Category: Lee YS]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:10:42 2008''
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[[Category: Park SS]]
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[[Category: Rhee S]]
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[[Category: Yang IS]]

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Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

PDB ID 1or0

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