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1ot5

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(New page: 200px<br /><applet load="1ot5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ot5, resolution 2.40&Aring;" /> '''The 2.4 Angstrom Cry...)
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[[Image:1ot5.gif|left|200px]]<br /><applet load="1ot5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ot5, resolution 2.40&Aring;" />
 
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'''The 2.4 Angstrom Crystal Sructure of Kex2 in complex with a peptidyl-boronic acid inhibitor'''<br />
 
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==Overview==
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==The 2.4 Angstrom Crystal Structure of Kex2 in complex with a peptidyl-boronic acid inhibitor==
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This paper reports the first structure of a member of the Kex2/furin, family of eukaryotic pro-protein processing proteases, which cleave sites, consisting of pairs or clusters of basic residues. Reported is the 2.4 A, resolution crystal structure of the two-domain protein ssKex2 in complex, with an Ac-Ala-Lys-boroArg inhibitor (R = 20.9%, R(free) = 24.5%). The, Kex2 proteolytic domain is similar in its global fold to the, subtilisin-like superfamily of degradative proteases. Analysis of the, complex provides a structural basis for the extreme selectivity of this, enzyme family that has evolved from a nonspecific subtilisin-like, ancestor. The P-domain of ssKex2 has a novel jelly roll like fold, consisting of nine beta strands and may potentially be involved, along, with the buried Ca(2+) ion, in creating the highly determined binding site, for P(1) arginine.
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<StructureSection load='1ot5' size='340' side='right'caption='[[1ot5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ot5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OT5 FirstGlance]. <br>
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1OT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAG, CA and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Kexin Kexin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.61 3.4.21.61] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OT5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BOR:(1R)-1-AMINO-4-{[(E)-AMINO(IMINO)METHYL]AMINO}BUTYLBORONIC+ACID'>BOR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ot5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot5 OCA], [https://pdbe.org/1ot5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ot5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ot5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ot5 ProSAT]</span></td></tr>
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2.4 A resolution crystal structure of the prototypical hormone-processing protease Kex2 in complex with an Ala-Lys-Arg boronic acid inhibitor., Holyoak T, Wilson MA, Fenn TD, Kettner CA, Petsko GA, Fuller RS, Ringe D, Biochemistry. 2003 Jun 10;42(22):6709-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12779325 12779325]
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</table>
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[[Category: Kexin]]
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== Function ==
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[https://www.uniprot.org/uniprot/KEX2_YEAST KEX2_YEAST] Processing of precursors of alpha-factors and killer toxin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1ot5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ot5 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Fenn TD]]
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[[Category: Fenn, T.D.]]
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[[Category: Fuller RS]]
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[[Category: Fuller, R.S.]]
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[[Category: Holyoak T]]
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[[Category: Holyoak, T.]]
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[[Category: Kettner CA]]
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[[Category: Kettner, C.A.]]
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[[Category: Petsko GA]]
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[[Category: Petsko, G.A.]]
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[[Category: Ringe D]]
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[[Category: Ringe, D.]]
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[[Category: Wilson MA]]
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[[Category: Wilson, M.A.]]
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[[Category: ACE]]
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[[Category: CA]]
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[[Category: NAG]]
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[[Category: p-domain]]
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[[Category: peptidyl-boronic acid]]
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[[Category: serine protease]]
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[[Category: subtilisin fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:10:23 2007''
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Current revision

The 2.4 Angstrom Crystal Structure of Kex2 in complex with a peptidyl-boronic acid inhibitor

PDB ID 1ot5

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