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1out

From Proteopedia

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[[Image:1out.jpg|left|200px]]
 
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{{Structure
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==TROUT HEMOGLOBIN I==
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|PDB= 1out |SIZE=350|CAPTION= <scene name='initialview01'>1out</scene>, resolution 2.3&Aring;
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<StructureSection load='1out' size='340' side='right'caption='[[1out]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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<table><tr><td colspan='2'>[[1out]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OUT FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1out FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1out OCA], [https://pdbe.org/1out PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1out RCSB], [https://www.ebi.ac.uk/pdbsum/1out PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1out ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HBA1_ONCMY HBA1_ONCMY] Involved in oxygen transport from gills to the various peripheral tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/1out_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1out ConSurf].
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<div style="clear:both"></div>
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'''TROUT HEMOGLOBIN I'''
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==See Also==
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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We have determined the X-ray crystallographic structure of trout Hb I in both the deoxy and carbonmonoxy forms to resolution limits of 2.3 angstroms and 2.5 angstroms, respectively. The overall fold of the molecule is highly similar to that of human HbA despite the low level of sequence identity between these proteins. Trout Hb I is unusual in displaying almost no pH dependence of oxygen binding affinity, and (at most) very weak interactions with heterotropic effector ligands such as organic phosphates. Comparison of the two quaternary states of the protein indicates how such effects are minimised and how the low-affinity T state of the protein is stabilised in the absence of heterotropic interactions.
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[[Category: Large Structures]]
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==About this Structure==
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1OUT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUT OCA].
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==Reference==
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The crystal structures of trout Hb I in the deoxy and carbonmonoxy forms., Tame JR, Wilson JC, Weber RE, J Mol Biol. 1996 Jun 21;259(4):749-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8683580 8683580]
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[[Category: Oncorhynchus mykiss]]
[[Category: Oncorhynchus mykiss]]
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[[Category: Protein complex]]
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[[Category: Tame J]]
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[[Category: Tame, J.]]
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[[Category: Wilson J]]
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[[Category: Wilson, J.]]
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[[Category: ACE]]
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[[Category: HEM]]
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[[Category: erythrocyte]]
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[[Category: heme]]
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[[Category: oxygen transport]]
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[[Category: respiratory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:15:55 2008''
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Current revision

TROUT HEMOGLOBIN I

PDB ID 1out

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