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1ouw

From Proteopedia

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[[Image:1ouw.png|left|200px]]
 
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{{STRUCTURE_1ouw| PDB=1ouw | SCENE= }}
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==Crystal structure of Calystegia sepium agglutinin==
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<StructureSection load='1ouw' size='340' side='right'caption='[[1ouw]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ouw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OUW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ouw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ouw OCA], [https://pdbe.org/1ouw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ouw RCSB], [https://www.ebi.ac.uk/pdbsum/1ouw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ouw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECC_CALSE LECC_CALSE] Mannose-binding lectin (PubMed:9111143, PubMed:18266762, PubMed:14561768, PubMed:26971576, PubMed:28973127). Preferentially binds mannose at concentrations ranging between 5 and 25 mM, but binds also glucose. Has a marked preference for methylated sugar derivatives, such as alpha-MeMan and alpha-MeGlc, at concentration down to 5 mM (PubMed:14561768). Binds to N-glycans, but not to glycolipid-type or other type of glycans (PubMed:28973127). Binds N-linked high-mannose-type glycans (PubMed:18266762, PubMed:28973127). Has a preference for smaller (Man(2)-Man(6)) high-mannose-type glycans to larger (Man(7)-Man(9)) ones. Recognizes both alpha1-6 extended and alpha1-3 extended monoantennary glycans. The addition of alpha1-2Man to the Man-alpha1-3Man-beta branch results in a significant loss of affinity, but beta1-2GlcNAc has some affinity. Has less affinity for biantennary glycans (PubMed:18266762). However, affinity is significant for the biantennary complex-type N-glycans with bisecting GlcNAc (PubMed:18266762, PubMed:26971576, PubMed:28973127). No affinity is observed for tri- and tetra-antennary glycans (PubMed:18266762). Binds bisected glycans of the mouse brain. Selectively binds to bisecting N-glycans which are in back-fold conformation, and does not favor a glycan with an extend conformation (PubMed:26971576). Has hemagglutinating activity against rabbit erythrocytes at 0.3 ug/ml and against trypsin-treated human erythrocytes at 5 ug/ml. Has mitogenic activity in murine cells (PubMed:9111143).<ref>PMID:14561768</ref> <ref>PMID:18266762</ref> <ref>PMID:26971576</ref> <ref>PMID:28973127</ref> <ref>PMID:9111143</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ou/1ouw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ouw ConSurf].
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<div style="clear:both"></div>
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===Crystal structure of Calystegia sepium agglutinin===
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==See Also==
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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{{ABSTRACT_PUBMED_14561768}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1ouw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUW OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:014561768</ref><references group="xtra"/>
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[[Category: Calystegia sepium]]
[[Category: Calystegia sepium]]
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[[Category: Astoul, C H.]]
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[[Category: Large Structures]]
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[[Category: Barre, A.]]
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[[Category: Astoul CH]]
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[[Category: Bourne, Y.]]
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[[Category: Barre A]]
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[[Category: Damme, E J.M van.]]
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[[Category: Bourne Y]]
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[[Category: Peumans, W J.]]
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[[Category: Peumans WJ]]
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[[Category: Roig-Zamboni, V.]]
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[[Category: Roig-Zamboni V]]
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[[Category: Rouge, P.]]
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[[Category: Rouge P]]
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[[Category: Agglutinin]]
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[[Category: Van Damme EJM]]
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[[Category: Beta-prism fold]]
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[[Category: Jacalin-related]]
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[[Category: Lectin]]
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[[Category: Mannose-binding]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of Calystegia sepium agglutinin

PDB ID 1ouw

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