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1oxw

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[[Image:1oxw.gif|left|200px]]<br /><applet load="1oxw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oxw, resolution 2.20&Aring;" />
 
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'''The Crystal Structure of SeMet Patatin'''<br />
 
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==Overview==
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==The Crystal Structure of SeMet Patatin==
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Patatin is a nonspecific lipid acyl hydrolase that accounts for approximately 40% of the total soluble protein in mature potato tubers, and it has potent insecticidal activity against the corn rootworm. We determined the X-ray crystal structure of a His-tagged variant of an isozyme of patatin, Pat17, to 2.2 A resolution, employing SeMet multiwavelength anomalous dispersion (MAD) phasing methods. The patatin crystal structure has three molecules in the asymmetric unit, an R-factor of 22.0%, and an R(free) of 27.2% (for 10% of the data not included in the refinement) and includes 498 water molecules. The structure notably revealed that patatin has a Ser-Asp catalytic dyad and an active site like that of human cytosolic phospholipase A(2) (cPLA(2)) [Dessen, A., et al. (1999) Cell 97, 349-360]. In addition, patatin has a folding topology related to that of the catalytic domain of cPLA(2) and unlike the canonical alpha/beta-hydrolase fold. The structure confirms our site-directed mutagenesis and bioactivity data that initially suggested patatin possessed a Ser-Asp catalytic dyad. Alanine-scanning mutagenesis revealed that Ser77 and Asp215 were critical for both esterase and bioactivity, consistent with prior work implicating a Ser residue [Strickland, J. H., et al. (1995) Plant Physiol. 109, 667-674] and a Ser-Asp dyad [Hirschberg, H. J. H. B., et al. (2001) Eur. J. Biochem. 268, 5037-5044] in patatin's catalytic activity. The crystal structure aids the understanding of other structure-function relationships in patatin. Patatin does not display interfacial activation, a hallmark feature of lipases, and this is likely due to the fact that it lacks a flexible lid that can shield the active site.
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<StructureSection load='1oxw' size='340' side='right'caption='[[1oxw]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oxw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_cardiophyllum Solanum cardiophyllum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXW FirstGlance]. <br>
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1OXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_cardiophyllum Solanum cardiophyllum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxw OCA], [https://pdbe.org/1oxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxw RCSB], [https://www.ebi.ac.uk/pdbsum/1oxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxw ProSAT]</span></td></tr>
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The crystal structure, mutagenesis, and activity studies reveal that patatin is a lipid acyl hydrolase with a Ser-Asp catalytic dyad., Rydel TJ, Williams JM, Krieger E, Moshiri F, Stallings WC, Brown SM, Pershing JC, Purcell JP, Alibhai MF, Biochemistry. 2003 Jun 10;42(22):6696-708. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12779324 12779324]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/PAT17_SOLCD PAT17_SOLCD]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oxw ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Solanum cardiophyllum]]
[[Category: Solanum cardiophyllum]]
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[[Category: Alibhai, M F.]]
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[[Category: Alibhai MF]]
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[[Category: Brown, S M.]]
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[[Category: Brown SM]]
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[[Category: Krieger, E.]]
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[[Category: Krieger E]]
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[[Category: Moshiri, F.]]
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[[Category: Moshiri F]]
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[[Category: Pershing, J C.]]
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[[Category: Pershing JC]]
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[[Category: Purcell, J P.]]
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[[Category: Purcell JP]]
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[[Category: Rydel, T J.]]
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[[Category: Rydel TJ]]
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[[Category: Stallings, W C.]]
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[[Category: Stallings WC]]
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[[Category: Williams, J M.]]
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[[Category: Williams JM]]
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[[Category: alpha/beta class fold with approximately three layers]]
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[[Category: alpha/beta/alpha in content. possesses a central six-stranded beta sheet with alpha-helices front & back]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:54 2008''
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Current revision

The Crystal Structure of SeMet Patatin

PDB ID 1oxw

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