This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1p28

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:52, 17 April 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1p28.gif|left|200px]]
 
-
{{Structure
+
==The crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one.==
-
|PDB= 1p28 |SIZE=350|CAPTION= <scene name='initialview01'>1p28</scene>, resolution 1.70&Aring;
+
<StructureSection load='1p28' size='340' side='right'caption='[[1p28]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=HBS:S,3-HYDROXYBUTAN-2-ONE'>HBS</scene> and <scene name='pdbligand=HBR:R,3-HYDROXYBUTAN-2-ONE'>HBR</scene>
+
<table><tr><td colspan='2'>[[1p28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhyparobia_maderae Rhyparobia maderae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P28 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HBR:R,3-HYDROXYBUTAN-2-ONE'>HBR</scene>, <scene name='pdbligand=HBS:S,3-HYDROXYBUTAN-2-ONE'>HBS</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p28 OCA], [https://pdbe.org/1p28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p28 RCSB], [https://www.ebi.ac.uk/pdbsum/1p28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p28 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''The crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one.'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/Q8MTC1_RHYMA Q8MTC1_RHYMA]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Pheromone-binding proteins (PBPs) are small helical proteins found in sensorial organs, particularly in the antennae, of moth and other insect species. They were proposed to solubilize and carry the hydrophobic pheromonal compounds through the antennal lymph to receptors, participating thus in the peri-receptor events of signal transduction. The x-ray structure of Bombyx mori PBP (BmorPBP), from male antennae, revealed a six-helix fold forming a cavity that contains the pheromone bombykol. We have identified a PBP (LmaPBP) from the cockroach Leucophaea maderae in the antennae of the females, the gender attracted by pheromones in this species. Here we report the crystal structure of LmaPBP alone or in complex with a fluorescent reporter (amino-naphthalen sulfonate, ANS) or with a component of the pheromonal blend, 3-hydroxy-butan-2-one. Both compounds bind in the internal cavity of LmaPBP, which is more hydrophilic than BmorPBP cavity. LmaPBP structure ends just after the sixth helix (helix F). BmorPBP structure extends beyond the sixth helix with a stretch of residues elongated at neutral pH and folding as a seventh internalized helix at low pH. These differences between LmaPBP and BmorPBP structures suggest that different binding and release mechanism may be adapted to the hydrophilicity or hydrophobicity of the pheromonal ligand.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p2/1p28_consurf.spt"</scriptWhenChecked>
-
1P28 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leucophaea_maderae Leucophaea maderae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P28 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
The crystal structure of a cockroach pheromone-binding protein suggests a new ligand binding and release mechanism., Lartigue A, Gruez A, Spinelli S, Riviere S, Brossut R, Tegoni M, Cambillau C, J Biol Chem. 2003 Aug 8;278(32):30213-8. Epub 2003 May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12766173 12766173]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p28 ConSurf].
-
[[Category: Leucophaea maderae]]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
__TOC__
-
[[Category: Brossut, R.]]
+
</StructureSection>
-
[[Category: Cambillau, C.]]
+
[[Category: Large Structures]]
-
[[Category: Gruez, A.]]
+
[[Category: Rhyparobia maderae]]
-
[[Category: Lartigue, A.]]
+
[[Category: Brossut R]]
-
[[Category: Riviere, S.]]
+
[[Category: Cambillau C]]
-
[[Category: Spinelli, S.]]
+
[[Category: Gruez A]]
-
[[Category: Tegoni, M.]]
+
[[Category: Lartigue A]]
-
[[Category: HBR]]
+
[[Category: Riviere S]]
-
[[Category: HBS]]
+
[[Category: Spinelli S]]
-
[[Category: 3-hydroxy-butan-2-one]]
+
[[Category: Tegoni M]]
-
[[Category: pheromone binding protein]]
+
-
[[Category: transport protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:18:48 2008''
+

Current revision

The crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one.

PDB ID 1p28

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p28"
Personal tools