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1p49

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(New page: 200px<br /> <applet load="1p49" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p49, resolution 2.60&Aring;" /> '''Structure of Human ...)
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[[Image:1p49.gif|left|200px]]<br />
 
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<applet load="1p49" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p49, resolution 2.60&Aring;" />
 
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'''Structure of Human Placental Estrone/DHEA Sulfatase'''<br />
 
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==Overview==
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==Structure of Human Placental Estrone/DHEA Sulfatase==
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Estrone sulfatase (ES; 562 amino acids), one of the key enzymes, responsible for maintaining high levels of estrogens in breast tumor, cells, is associated with the membrane of the endoplasmic reticulum (ER)., The structure of ES, purified from the microsomal fraction of human, placentas, has been determined at 2.60-A resolution by x-ray, crystallography. This structure shows a domain consisting of two, antiparallel alpha-helices that protrude from the roughly spherical, molecule, thereby giving the molecule a "mushroom-like" shape. These, highly hydrophobic helices, each about 40 A long, are capable of, traversing the membrane, thus presumably anchoring the functional domain, on the membrane surface facing the ER lumen. The location of the, transmembrane domain is such that the opening to the active site, buried, deep in a cavity of the "gill" of the "mushroom," rests near the membrane, surface, thereby suggesting a role of the lipid bilayer in catalysis. This, simple architecture could be a prototype utilized by the ER membrane in, dictating the form and the function of ER-resident enzymes.
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<StructureSection load='1p49' size='340' side='right'caption='[[1p49]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P49 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALS:(3S)-3-(SULFOOXY)-L-SERINE'>ALS</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p49 OCA], [https://pdbe.org/1p49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p49 RCSB], [https://www.ebi.ac.uk/pdbsum/1p49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p49 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/STS_HUMAN STS_HUMAN] Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:[https://omim.org/entry/308100 308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.<ref>PMID:1539590</ref> <ref>PMID:9252398</ref> <ref>PMID:10679952</ref> <ref>PMID:10844566</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/STS_HUMAN STS_HUMAN] Conversion of sulfated steroid precursors to estrogens during pregnancy.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/1p49_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p49 ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Ichthyosis, X-linked OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=308100 308100]], Placental steroid sulfatase deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=308100 308100]]
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*[[Sulfatase 3D structures|Sulfatase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1P49 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BOG, NAG, CA and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Steryl-sulfatase Steryl-sulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.2 3.1.6.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P49 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structure of human estrone sulfatase suggests functional roles of membrane association., Hernandez-Guzman FG, Higashiyama T, Pangborn W, Osawa Y, Ghosh D, J Biol Chem. 2003 Jun 20;278(25):22989-97. Epub 2003 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12657638 12657638]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Steryl-sulfatase]]
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[[Category: Ghosh D]]
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[[Category: Ghosh, D.]]
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[[Category: Hernandez-Guzman FG]]
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[[Category: Hernandez-Guzman, F.G.]]
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[[Category: Higashiyama T]]
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[[Category: Higashiyama, T.]]
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[[Category: Osawa Y]]
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[[Category: Osawa, Y.]]
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[[Category: Pangborn W]]
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[[Category: Pangborn, W.]]
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[[Category: BOG]]
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[[Category: CA]]
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[[Category: NAG]]
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[[Category: PO4]]
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[[Category: dehydroepiandrosterone sulfate]]
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[[Category: endoplasmic reticulum membrane-bound]]
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[[Category: estrone sulfate]]
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[[Category: human placental enzyme]]
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[[Category: steroid biosynthesis]]
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[[Category: steroid sulfatase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:39:52 2007''
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Current revision

Structure of Human Placental Estrone/DHEA Sulfatase

PDB ID 1p49

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