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1pbx

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(New page: 200px<br /> <applet load="1pbx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pbx, resolution 2.5&Aring;" /> '''HAEMOGLOBIN OF THE A...)
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[[Image:1pbx.gif|left|200px]]<br />
 
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<applet load="1pbx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pbx, resolution 2.5&Aring;" />
 
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'''HAEMOGLOBIN OF THE ANTARCTIC FISH PAGOTHENIA BERNACCHII: AMINO ACID SEQUENCE, OXYGEN EQUILIBRIA AND CRYSTAL STRUCTURE OF ITS CARBONMONOXY DERIVATIVE'''<br />
 
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==Overview==
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==HAEMOGLOBIN OF THE ANTARCTIC FISH PAGOTHENIA BERNACCHII: AMINO ACID SEQUENCE, OXYGEN EQUILIBRIA AND CRYSTAL STRUCTURE OF ITS CARBONMONOXY DERIVATIVE==
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The Antarctic fish Pagothenia bernacchii has one major haemoglobin, Hb1, (over 95% of the total blood content). Hb1 has a strong alkaline Bohr, effect and at low pH exhibits the reduced ligand affinity and, co-operativity that comprise the Root effect. We have determined the, complete amino acid sequence of P. bernacchii Hb1 and also the structure, of its carbonmonoxy derivative by X-ray crystallography, to a resolution, of 2.5 A. The crystallographic R-factor of the refined structure is 18%., The three-dimensional structure of this fish haemoglobin is similar to, that of human haemoglobin A, with a root-mean-square difference in, main-chain atom positions of 1.4 A after superimposition of the two, structures, despite only 48% homology of their amino acid sequences, (including insertion of a single residue in the CD region of the fish, alpha-chain). Large structural differences occur only at the N and C, termini of both the alpha- and beta-chains. Neither these nor other, smaller structural differences provide any obvious explanation of the Root, effect of this or other fish haemoglobins.
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<StructureSection load='1pbx' size='340' side='right'caption='[[1pbx]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pbx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PBX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pbx OCA], [https://pdbe.org/1pbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pbx RCSB], [https://www.ebi.ac.uk/pdbsum/1pbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pbx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HBA_TREBE HBA_TREBE] Involved in oxygen transport from gills to the various peripheral tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/1pbx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pbx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii] with HEM and CMO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PBX OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Haemoglobin of the antarctic fish Pagothenia bernacchii. Amino acid sequence, oxygen equilibria and crystal structure of its carbonmonoxy derivative., Camardella L, Caruso C, D'Avino R, di Prisco G, Rutigliano B, Tamburrini M, Fermi G, Perutz MF, J Mol Biol. 1992 Mar 20;224(2):449-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1560461 1560461]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Trematomus bernacchii]]
[[Category: Trematomus bernacchii]]
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[[Category: Fermi, G.]]
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[[Category: Fermi G]]
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[[Category: CMO]]
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[[Category: HEM]]
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[[Category: oxygen transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:15:39 2007''
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Current revision

HAEMOGLOBIN OF THE ANTARCTIC FISH PAGOTHENIA BERNACCHII: AMINO ACID SEQUENCE, OXYGEN EQUILIBRIA AND CRYSTAL STRUCTURE OF ITS CARBONMONOXY DERIVATIVE

PDB ID 1pbx

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