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1pcp

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(New page: 200px<br /><applet load="1pcp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pcp" /> '''SOLUTION STRUCTURE OF A TREFOIL-MOTIF-CONTAI...)
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[[Image:1pcp.gif|left|200px]]<br /><applet load="1pcp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pcp" />
 
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'''SOLUTION STRUCTURE OF A TREFOIL-MOTIF-CONTAINING CELL GROWTH FACTOR, PORCINE SPASMOLYTIC PROTEIN'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF A TREFOIL-MOTIF-CONTAINING CELL GROWTH FACTOR, PORCINE SPASMOLYTIC PROTEIN==
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The porcine spasmolytic protein (pSP) is a 106-residue cell growth factor, that typifies a family of eukaryotic proteins that contain at least one, copy of an approximately 40-amino acid protein domain known as the trefoil, motif. In fact, pSP contains two highly homologous trefoil domains. We, have determined the complete three-dimensional solution structure of pSP, by using a combination of two- and three-dimensional 1H NMR spectroscopy, and distance geometry calculations. pSP is a relatively elongated, molecule, consisting of two compact globular domains joined via a small, interface. The protein's two trefoil domains adopt the same tertiary, structure and contain a core C-terminal two-stranded antiparallel, beta-sheet, preceded by a 6-residue helix that packs against the, N-terminal beta-strand. The remainder of the protein backbone is taken up, by two short loops that lie on either side of the beta-hairpin and are, linked by an extended region that wraps around the C-terminal beta-strand., The topology of the protein backbone observed for the trefoil domains in, pSP represents an unusual polypeptide fold. A striking feature of both, trefoil domains is a surface patch formed from five conserved residues, that have no obvious structural role. The two patches are located at the, far ends of the protein molecule, and we propose that these residues form, at least part of the receptor binding site, or sites, on pSP.
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<StructureSection load='1pcp' size='340' side='right'caption='[[1pcp]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PCP FirstGlance]. <br>
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1PCP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PCP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pcp OCA], [https://pdbe.org/1pcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pcp RCSB], [https://www.ebi.ac.uk/pdbsum/1pcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pcp ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of a trefoil-motif-containing cell growth factor, porcine spasmolytic protein., Carr MD, Bauer CJ, Gradwell MJ, Feeney J, Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2206-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8134374 8134374]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/TFF2_PIG TFF2_PIG] Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/1pcp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pcp ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Bauer, C.J.]]
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[[Category: Bauer CJ]]
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[[Category: Carr, M.D.]]
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[[Category: Carr MD]]
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[[Category: Feeney, J.]]
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[[Category: Feeney J]]
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[[Category: Gradwell, M.J.]]
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[[Category: Gradwell MJ]]
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[[Category: growth factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:41:36 2007''
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Current revision

SOLUTION STRUCTURE OF A TREFOIL-MOTIF-CONTAINING CELL GROWTH FACTOR, PORCINE SPASMOLYTIC PROTEIN

PDB ID 1pcp

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