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1plg

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(New page: 200px<br /><applet load="1plg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1plg, resolution 2.8&Aring;" /> '''EVIDENCE FOR THE EXTE...)
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[[Image:1plg.gif|left|200px]]<br /><applet load="1plg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1plg, resolution 2.8&Aring;" />
 
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'''EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID'''<br />
 
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==Overview==
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==EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID==
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The antigen binding fragment from an IgG2a kappa murine monoclonal, antibody with specificity for alpha-(2--&gt;8)-linked sialic acid polymers, has been prepared and crystallized in the absence of hapten. Crystals were, grown by vapor diffusion equilibrium with 16-18% polyethylene glycol 4000, solutions. The structure was solved by molecular replacement methods and, refined to a conventional R factor of 0.164 for data to 2.8 A. The binding, site is observed to display a shape and distribution of charges that is, complementary to that of the predicted conformation of the oligosaccharide, epitope. A thermodynamic description of ligand binding has been compiled, for oligosaccharides ranging in length from 9 to 41 residues, and the data, for the largest ligand has been used in a novel way to estimate the size, of the antigen binding site. A model of antigen binding is presented that, satisfies this thermodynamic data, as well as a previously reported, requirement of conformational specificity of the oligosaccharide. X-ray, crystallographic and thermodynamic evidence are consistent with a binding, site that accommodates at least eight sialic acid residues.
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<StructureSection load='1plg' size='340' side='right'caption='[[1plg]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1plg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PLG FirstGlance]. <br>
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1PLG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PLG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1plg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plg OCA], [https://pdbe.org/1plg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1plg RCSB], [https://www.ebi.ac.uk/pdbsum/1plg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1plg ProSAT]</span></td></tr>
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==Reference==
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</table>
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Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2--&gt;8)-polysialic acid., Evans SV, Sigurskjold BW, Jennings HJ, Brisson JR, To R, Tse WC, Altman E, Frosch M, Weisgerber C, Kratzin HD, et al., Biochemistry. 1995 May 23;34(20):6737-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7538787 7538787]
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== Function ==
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[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/1plg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1plg ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Altman E]]
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[[Category: Altman, E.]]
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[[Category: Bitter-Suermann D]]
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[[Category: Bitter-Suermann, D.]]
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[[Category: Brisson J-R]]
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[[Category: Brisson, J.R.]]
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[[Category: Bundle DR]]
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[[Category: Bundle, D.R.]]
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[[Category: Evans SV]]
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[[Category: Evans, S.V.]]
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[[Category: Frosch M]]
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[[Category: Frosch, M.]]
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[[Category: Jennings HJ]]
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[[Category: Jennings, H.J.]]
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[[Category: Klebert S]]
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[[Category: Klebert, S.]]
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[[Category: Kratzin H]]
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[[Category: Kratzin, H.]]
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[[Category: Rose DR]]
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[[Category: Rose, D.R.]]
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[[Category: Sigurskjold BW]]
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[[Category: Sigurskjold, B.W.]]
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[[Category: To R]]
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[[Category: To, R.]]
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[[Category: Tse WC]]
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[[Category: Tse, W.C.]]
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[[Category: Vaesen M]]
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[[Category: Vaesen, M.]]
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[[Category: Weisgerber C]]
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[[Category: Weisgerber, C.]]
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[[Category: Young NM]]
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[[Category: Young, N.M.]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:39:31 2007''
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Current revision

EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID

PDB ID 1plg

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