This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ps2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:58, 17 April 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ps2.jpg|left|200px]]<br /><applet load="1ps2" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ps2" />
 
-
'''HIGH RESOLUTION NMR SOLUTION STRUCTURE OF HUMAN PS2, 19 STRUCTURES'''<br />
 
-
==Overview==
+
==HIGH RESOLUTION NMR SOLUTION STRUCTURE OF HUMAN PS2, 19 STRUCTURES==
-
pNR-2/pS2 is a 60 residue extracellular protein, which was originally, discovered in human breast cancer cells, and subsequently found in other, tumours and normal gastric epithelial cells. We have determined the, three-dimensional solution structure of a C58S mutant of human pNR-2/pS2, using 639 distance and 137 torsion angle constraints obtained from, analysis of multidimensional NMR spectra. A series of simulated annealing, calculations resulted in the unambiguous determination of the protein's, disulphide bonding pattern and produced a family of 19 structures, consistent with the constraints. The peptide contains a single "trefoil", sequence motif, a region of about 40 residues with a characteristic, sequence pattern, which has been found, either singly or as a repeat, in, about a dozen extracellular proteins. The trefoil domain contains three, disulphide bonds, whose 1-5, 2-4 and 3-6 cysteine pairings form the, structure into three closely packed loops with only a small amount of, secondary structure, which consists of a short alpha-helix packed against, a two-stranded antiparallel beta-sheet. The structure of the domain is, very similar to those of the two trefoil domains that occur in porcine, spasmolytic polypeptide (PSP), the only member of the trefoil family whose, three-dimensional structure has been previously determined. Outside the, trefoil domain, which forms the compact "head" of the molecule, the N and, C-terminal strands are closely associated, forming an extended "tail", which has some beta-sheet character for part of its length and which, becomes more disordered towards the termini as indicated by (15)N{(1)H}, NOEs. We have considered the structural implications of the possible, formation of a native C58-C58 disulphide-bonded homodimer. Comparison of, the surface features of pNR-2/pS2 and PSP, and consideration of the, sequences of the other human trefoil domains in the light of these, structures, illuminates the possible role of specific residues in, ligand/receptor binding.
+
<StructureSection load='1ps2' size='340' side='right'caption='[[1ps2]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1ps2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PS2 FirstGlance]. <br>
-
1PS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PS2 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ps2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ps2 OCA], [https://pdbe.org/1ps2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ps2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ps2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ps2 ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
High-resolution solution structure of human pNR-2/pS2: a single trefoil motif protein., Polshakov VI, Williams MA, Gargaro AR, Frenkiel TA, Westley BR, Chadwick MP, May FE, Feeney J, J Mol Biol. 1997 Mar 28;267(2):418-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9096235 9096235]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/TFF1_HUMAN TFF1_HUMAN] Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.<ref>PMID:16308573</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1ps2_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ps2 ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Feeney, J.]]
+
[[Category: Feeney J]]
-
[[Category: Gargaro, A.R.]]
+
[[Category: Gargaro AR]]
-
[[Category: Polshakov, V.I.]]
+
[[Category: Polshakov VI]]
-
[[Category: Williams, M.A.]]
+
[[Category: Williams MA]]
-
[[Category: cell motility]]
+
-
[[Category: growth factor]]
+
-
[[Category: signal]]
+
-
[[Category: trefoil domain]]
+
-
[[Category: tumor suppressor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:41:15 2008''
+

Current revision

HIGH RESOLUTION NMR SOLUTION STRUCTURE OF HUMAN PS2, 19 STRUCTURES

PDB ID 1ps2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ps2"
Personal tools