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1q16

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(New page: 200px<br /><applet load="1q16" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q16, resolution 1.90&Aring;" /> '''Crystal structure of...)
Current revision (06:00, 17 April 2024) (edit) (undo)
 
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[[Image:1q16.gif|left|200px]]<br /><applet load="1q16" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q16, resolution 1.90&Aring;" />
 
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'''Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli'''<br />
 
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==Overview==
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==Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli==
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The facultative anaerobe Escherichia coli is able to assemble specific, respiratory chains by synthesis of appropriate dehydrogenases and, reductases in response to the availability of specific substrates. Under, anaerobic conditions in the presence of nitrate, E. coli synthesizes the, cytoplasmic membrane-bound quinol-nitrate oxidoreductase (nitrate, reductase A; NarGHI), which reduces nitrate to nitrite and forms part of a, redox loop generating a proton-motive force. We present here the crystal, structure of NarGHI at a resolution of 1.9 A. The NarGHI structure, identifies the number, coordination scheme and environment of the, redox-active prosthetic groups, a unique coordination of the molybdenum, atom, the first structural evidence for the role of an open bicyclic form, of the molybdo-bis(molybdopterin guanine dinucleotide) (Mo-bisMGD), cofactor in the catalytic mechanism and a novel fold of the membrane, anchor subunit. Our findings provide fundamental molecular details for, understanding the mechanism of proton-motive force generation by a redox, loop.
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<StructureSection load='1q16' size='340' side='right'caption='[[1q16]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q16]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q16 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PH:1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE'>3PH</scene>, <scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=AGA:(1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL+OCTANOATE'>AGA</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MD1:PHOSPHORIC+ACID+4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL+ESTER+GUANYLATE+ESTER'>MD1</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q16 OCA], [https://pdbe.org/1q16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q16 RCSB], [https://www.ebi.ac.uk/pdbsum/1q16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q16 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NARH_ECOLI NARH_ECOLI] The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q16_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q16 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Q16 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MD1, 6MO, HEM, SF4, F3S, AGA and 3PH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q16 OCA].
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*[[Nitrate reductase|Nitrate reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A., Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC, Nat Struct Biol. 2003 Sep;10(9):681-7. Epub 2003 Aug 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12910261 12910261]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Nitrate reductase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Bertero MG]]
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[[Category: Bertero, M.G.]]
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[[Category: Strynadka NCJ]]
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[[Category: Strynadka, N.C.J.]]
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[[Category: 3PH]]
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[[Category: 6MO]]
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[[Category: AGA]]
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[[Category: F3S]]
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[[Category: HEM]]
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[[Category: MD1]]
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[[Category: SF4]]
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[[Category: electron-transfer]]
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[[Category: membrane protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:17:47 2007''
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Current revision

Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli

PDB ID 1q16

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