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1qj3

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(New page: 200px<br /> <applet load="1qj3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qj3, resolution 2.7&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1qj3.gif|left|200px]]<br />
 
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<applet load="1qj3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qj3, resolution 2.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF 7,8-DIAMINOPELARGONIC ACID SYNTHASE IN COMPLEX WITH 7-KETO-8-AMINOPELARGONIC ACID'''<br />
 
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==Overview==
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==Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid==
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The three-dimensional structure of diaminopelargonic acid synthase, a, vitamin B6-dependent enzyme in the pathway of the biosynthesis of biotin, has been determined to 1.8 A resolution by X-ray crystallography. The, structure was solved by multi-wavelength anomalous diffraction techniques, using a crystal derivatized with mercury ions. The protein model has been, refined to a crystallographic R -value of 17.5% (R -free 22.6%). Each, enzyme subunit consists of two domains, a large domain (residues 50-329), containing a seven-stranded predominantly parallel beta-sheet, surrounded, by alpha-helices, and a small domain comprising residues 1-49 and 330-429., Two subunits, related by a non-crystallographic dyad in the crystals, form, the homodimeric molecule, which contains two equal active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10452893 (full description)]]
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<StructureSection load='1qj3' size='340' side='right'caption='[[1qj3]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QJ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KAP:7-KETO-8-AMINOPELARGONIC+ACID'>KAP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qj3 OCA], [https://pdbe.org/1qj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1qj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qj3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BIOA_ECOLI BIOA_ECOLI] Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.<ref>PMID:1092681</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qj/1qj3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qj3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1QJ3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NA, PLP and KAP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.62 2.6.1.62]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJ3 OCA]].
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*[[7%2C8-diaminopelargonic acid synthase|7%2C8-diaminopelargonic acid synthase]]
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*[[7%2C8-diaminopelargonic acid synthase 3D structures|7%2C8-diaminopelargonic acid synthase 3D structures]]
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==Reference==
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== References ==
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Crystal structure of diaminopelargonic acid synthase: evolutionary relationships between pyridoxal-5'-phosphate-dependent enzymes., Kack H, Sandmark J, Gibson K, Schneider G, Lindqvist Y, J Mol Biol. 1999 Aug 27;291(4):857-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10452893 10452893]
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<references/>
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[[Category: Escherichia coli]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Gibson, K.J.]]
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[[Category: Large Structures]]
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[[Category: Kaeck, H.]]
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[[Category: Gibson KJ]]
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[[Category: Lindqvist, Y.]]
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[[Category: Kaeck H]]
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[[Category: Sandmark, J.]]
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[[Category: Lindqvist Y]]
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[[Category: Schneider, G.]]
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[[Category: Sandmark J]]
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[[Category: KAP]]
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[[Category: Schneider G]]
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[[Category: NA]]
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[[Category: PLP]]
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[[Category: aminotransferase]]
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[[Category: biotin biosynthesis]]
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[[Category: pyridoxal-5'-phosphate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:24:33 2007''
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Current revision

Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid

PDB ID 1qj3

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