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1qjd

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[[Image:1qjd.gif|left|200px]]<br />
 
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<applet load="1qjd" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qjd, resolution 1.80&Aring;" />
 
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'''FLAVOCYTOCHROME C3 FROM SHEWANELLA FRIGIDIMARINA'''<br />
 
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==Overview==
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==Flavocytochrome C3 from Shewanella frigidimarina==
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The 1.8 A resolution crystal structure of the tetraheme flavocytochrome, c3, Fcc3, provides the first mechanistic insight into respiratory fumarate, reductases or succinate dehydrogenases. The multi-redox center, three-domain protein shows a 40 A long 'molecular wire' allowing rapid, conduction of electrons through a new type of cytochrome domain onto the, active site flavin, driving the reduction of fumarate to succinate. In, this structure a malate-like molecule is trapped in the enzyme active, site. The interactions between this molecule and the enzyme suggest a, clear mechanism for fumarate reduction in which the substrate is polarized, and twisted, facilitating hydride transfer from the reduced flavin and, subsequent proton transfer. The enzyme active site in the oxidized form is, completely buried at the interface between the flavin-binding and the, clamp domains. Movement of the cytochrome and clamp domains is postulated, to allow release of the product.
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<StructureSection load='1qjd' size='340' side='right'caption='[[1qjd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qjd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_frigidimarina Shewanella frigidimarina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QJD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEO:MALATE+LIKE+INTERMEDIATE'>TEO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qjd OCA], [https://pdbe.org/1qjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qjd RCSB], [https://www.ebi.ac.uk/pdbsum/1qjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qjd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FRDA_SHEFN FRDA_SHEFN] Catalyzes fumarate reduction using artificial electron donors such as methyl viologen. The physiological reductant is unknown, but evidence indicates that flavocytochrome c participates in electron transfer from formate to fumarate and possibly also to trimethylamine oxide (TMAO). This enzyme is essentially unidirectional (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qj/1qjd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qjd ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1QJD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shewanella_frigidimarina Shewanella frigidimarina] with NA, HEC, FAD, TEO and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: SBS. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJD OCA].
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*[[Flavocytochrome 3D structures|Flavocytochrome 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural and mechanistic mapping of a unique fumarate reductase., Taylor P, Pealing SL, Reid GA, Chapman SK, Walkinshaw MD, Nat Struct Biol. 1999 Dec;6(12):1108-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10581550 10581550]
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[[Category: Large Structures]]
[[Category: Shewanella frigidimarina]]
[[Category: Shewanella frigidimarina]]
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[[Category: Single protein]]
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[[Category: Chapman SK]]
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[[Category: Chapman, S.K.]]
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[[Category: Pealing SL]]
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[[Category: Pealing, S.L.]]
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[[Category: Reid GA]]
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[[Category: Reid, G.A.]]
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[[Category: Taylor P]]
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[[Category: Taylor, P.]]
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[[Category: Walkinshaw MD]]
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[[Category: Walkinshaw, M.D.]]
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[[Category: FAD]]
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[[Category: GOL]]
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[[Category: HEC]]
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[[Category: NA]]
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[[Category: TEO]]
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[[Category: fumarate reductase]]
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[[Category: respiratory fumarate reductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:00:39 2007''
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Current revision

Flavocytochrome C3 from Shewanella frigidimarina

PDB ID 1qjd

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