This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qmi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:04, 17 April 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_1qmi| PDB=1qmi | SCENE= }}
 
-
===Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology===
 
-
{{ABSTRACT_PUBMED_10673421}}
 
-
==Function==
+
==Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology==
-
[[http://www.uniprot.org/uniprot/RTCA_ECOLI RTCA_ECOLI]] Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.<ref>PMID:9738023</ref>
+
<StructureSection load='1qmi' size='340' side='right'caption='[[1qmi]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1qmi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QMI FirstGlance]. <br>
-
[[1qmi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMI OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmi OCA], [https://pdbe.org/1qmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qmi RCSB], [https://www.ebi.ac.uk/pdbsum/1qmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qmi ProSAT]</span></td></tr>
-
<ref group="xtra">PMID:010673421</ref><references group="xtra"/><references/>
+
</table>
-
[[Category: Escherichia coli k-12]]
+
== Function ==
-
[[Category: RNA-3'-phosphate cyclase]]
+
[https://www.uniprot.org/uniprot/RTCA_ECOLI RTCA_ECOLI] Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.<ref>PMID:9738023</ref>
-
[[Category: Billy, E.]]
+
== Evolutionary Conservation ==
-
[[Category: Filipowicz, W.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Palm, G J.]]
+
Check<jmol>
-
[[Category: Wlodawer, A.]]
+
<jmolCheckbox>
-
[[Category: 2'3'cyclic phosphate rna]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qm/1qmi_consurf.spt"</scriptWhenChecked>
-
[[Category: Ligase]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qmi ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia coli K-12]]
 +
[[Category: Large Structures]]
 +
[[Category: Billy E]]
 +
[[Category: Filipowicz W]]
 +
[[Category: Palm GJ]]
 +
[[Category: Wlodawer A]]

Current revision

Crystal structure of RNA 3'-terminal phosphate cyclase, an ubiquitous enzyme with unusual topology

PDB ID 1qmi

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qmi"
Personal tools