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1qs8

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[[Image:1qs8.jpg|left|200px]]
 
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==Crystal structure of the P. vivax aspartic proteinase plasmepsin complexed with the inhibitor pepstatin A==
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The line below this paragraph, containing "STRUCTURE_1qs8", creates the "Structure Box" on the page.
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<StructureSection load='1qs8' size='340' side='right'caption='[[1qs8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qs8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax] and [https://en.wikipedia.org/wiki/Streptomyces_argenteolus_subsp._toyonakensis Streptomyces argenteolus subsp. toyonakensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QS8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=IVA:ISOVALERIC+ACID'>IVA</scene>, <scene name='pdbligand=STA:STATINE'>STA</scene></td></tr>
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{{STRUCTURE_1qs8| PDB=1qs8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qs8 OCA], [https://pdbe.org/1qs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qs8 RCSB], [https://www.ebi.ac.uk/pdbsum/1qs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qs8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O60989_PLAVI O60989_PLAVI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qs8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qs8 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE P. VIVAX ASPARTIC PROTEINASE PLASMEPSIN COMPLEXED WITH THE INHIBITOR PEPSTATIN A'''
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==See Also==
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*[[Plasmepsin|Plasmepsin]]
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__TOC__
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==Overview==
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</StructureSection>
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The malarial aspartic proteinases (plasmepsins) have been discovered in several species of Plasmodium, including all four of the human malarial pathogens. In P.falciparum, plasmepsins I, II, IV and HAP have been directly implicated in hemoglobin degradation during malaria infection, and are now considered targets for anti-malarial drug design. The plasmepsins are produced from inactive zymogens, proplasmepsins, having unusually long N-terminal prosegments of more than 120 amino acids. Structural and biochemical evidence suggests that the conversion process of proplasmepsins to plasmepsins differs substantially from the gastric and plant aspartic proteinases. Instead of blocking substrate access to a pre-formed active site, the prosegment enforces a conformation in which proplasmepsin cannot form a functional active site. We have determined crystal structures of plasmepsin and proplasmepsin from P.vivax. The three-dimensional structure of P.vivax plasmepsin is typical of the monomeric aspartic proteinases, and the structure of P.vivax proplasmepsin is similar to that of P.falciparum proplasmepsin II. A dramatic refolding of the mature N terminus and a large (18 degrees ) reorientation of the N-domain between P.vivax proplasmepsin and plasmepsin results in a severe distortion of the active site region of the zymogen relative to that of the mature enzyme. The present structures confirm that the mode of inactivation observed originally in P.falciparum proplasmepsin II, i.e. an incompletely formed active site, is a true structural feature and likely represents the general mode of inactivation of the related proplasmepsins.
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[[Category: Large Structures]]
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==About this Structure==
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1QS8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS8 OCA].
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==Reference==
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Structural insights into the activation of P. vivax plasmepsin., Bernstein NK, Cherney MM, Yowell CA, Dame JB, James MN, J Mol Biol. 2003 Jun 6;329(3):505-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12767832 12767832]
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[[Category: Plasmodium vivax]]
[[Category: Plasmodium vivax]]
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[[Category: Single protein]]
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[[Category: Streptomyces argenteolus subsp. toyonakensis]]
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[[Category: Bernstein, N Khazanovich.]]
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[[Category: Cherney MM]]
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[[Category: Cherney, M M.]]
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[[Category: Dame JB]]
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[[Category: Dame, J B.]]
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[[Category: James MNG]]
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[[Category: James, M N.G.]]
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[[Category: Khazanovich Bernstein N]]
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[[Category: Yowell, C A.]]
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[[Category: Yowell CA]]
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[[Category: Aspartic proteinase]]
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[[Category: Haemoglobinase]]
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[[Category: Hydrolase]]
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[[Category: Malaria]]
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[[Category: Pepstatin some]]
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[[Category: Plasmepsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:38:42 2008''
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Current revision

Crystal structure of the P. vivax aspartic proteinase plasmepsin complexed with the inhibitor pepstatin A

PDB ID 1qs8

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