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1r12
From Proteopedia
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==Native Aplysia ADP ribosyl cyclase== | ==Native Aplysia ADP ribosyl cyclase== | ||
| - | <StructureSection load='1r12' size='340' side='right' caption='[[1r12]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='1r12' size='340' side='right'caption='[[1r12]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1r12]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1r12]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R12 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r12 OCA], [https://pdbe.org/1r12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r12 RCSB], [https://www.ebi.ac.uk/pdbsum/1r12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r12 ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/NADA_APLCA NADA_APLCA] Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for calcium mobilization from endoplasmic reticulum. Also has cADPr hydrolase activity.<ref>PMID:11829748</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r12 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r12 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | ADP-ribosyl cyclase catalyzes the elimination of nicotinamide from NAD and cyclization to cADPR, a known second messenger in cellular calcium signaling pathways. We have determined to 2.0 A resolution the structure of Aplysia cyclase with ribose-5-phosphate bound covalently at C3' and with the base exchange substrate (BES), pyridylcarbinol, bound to the active site. In addition, further refinement at 2.4 A resolution of the structure of nicotinamide-bound cyclase, which was previously reported, reveals that ribose-5-phosphate is also covalently bound in this structure, and a second nicotinamide site was identified. The structures of native and mutant Glu179Ala cyclase were also solved to 1.7 and 2.0 A respectively. It is proposed that the second nicotinamide site serves to promote cyclization by clearing the active site of the nicotinamide byproduct. Moreover, a ribosylation mechanism can be proposed in which the cyclization reaction proceeds through a covalently bound intermediate. | ||
| - | + | ==See Also== | |
| - | + | *[[Cluster of Differentiation CD38|Cluster of Differentiation CD38]] | |
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== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Aplysia californica]] |
| - | [[Category: Graeff | + | [[Category: Large Structures]] |
| - | [[Category: Hao | + | [[Category: Graeff R]] |
| - | [[Category: Kriksunov | + | [[Category: Hao Q]] |
| - | [[Category: Lee | + | [[Category: Kriksunov IA]] |
| - | [[Category: Love | + | [[Category: Lee HC]] |
| - | [[Category: Munshi | + | [[Category: Love ML]] |
| - | [[Category: Szebenyi | + | [[Category: Munshi C]] |
| - | [[Category: Thiel | + | [[Category: Szebenyi DME]] |
| - | + | [[Category: Thiel DJ]] | |
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Current revision
Native Aplysia ADP ribosyl cyclase
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Categories: Aplysia californica | Large Structures | Graeff R | Hao Q | Kriksunov IA | Lee HC | Love ML | Munshi C | Szebenyi DME | Thiel DJ
