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1r8h

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(New page: 200px<br /><applet load="1r8h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r8h, resolution 1.90&Aring;" /> '''Comparison of the st...)
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[[Image:1r8h.gif|left|200px]]<br /><applet load="1r8h" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r8h, resolution 1.90&Aring;" />
 
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'''Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus'''<br />
 
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==Overview==
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==Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus==
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Human papillomaviruses (HPVS) that infect the genital tract can be divided, into two groups: high-risk HPV types, such as HPV 16 and HPV 18, are, associated with cancer, low-risk HPV types, such as HPV 6, are associated, with benign warts. In both high-risk and low-risk HPV types, the, papillomavirus E2 protein binds to four sites within the viral long, control region (LCR) and regulates viral gene expression. Here, we present, the crystal structure of the minimal DNA-binding domain (DBD) from the HPV, 6 E2 protein. We show that the HPV 6 E2 DBD is structurally more similar, to the HPV 18 and bovine papillomavirus type 1 (BPV1) E2 proteins than it, is to the HPV 16 E2 protein. Using gel retardation assays, we show that, the hierarchy of E2 sites within the HPV 16 and HPV 6 LCRs are different., However, despite these differences in structure and site preference, both, the HPV 16 and 6 E2 DBDs recognise an extended version of the consensus E2, binding site derived from studies of the BPV1 E2 protein. In both cases, the preferred binding site is 5'AACCGN(4)CGGTT3', where the additional, flanking base-pairs are in bold and N(4) represents a four base-pair, central spacer. Both of these HPV proteins bind preferentially to E2 sites, that contain an A:T-rich central spacer. We show that the preference for, an A:T-rich central spacer is due, at least in part, to the need to adopt, a DNA conformation that facilitates protein contacts with the flanking, base-pairs.
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<StructureSection load='1r8h' size='340' side='right'caption='[[1r8h]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r8h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_papillomavirus_type_6a Human papillomavirus type 6a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R8H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r8h OCA], [https://pdbe.org/1r8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r8h RCSB], [https://www.ebi.ac.uk/pdbsum/1r8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r8h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VE2_HPV6A VE2_HPV6A] E2 regulates viral transcription and DNA replication. It binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. It can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/1r8h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r8h ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1R8H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_1a Human papillomavirus type 1a] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R8H OCA].
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*[[Regulatory protein E2|Regulatory protein E2]]
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__TOC__
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==Reference==
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</StructureSection>
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Comparison of the structure and DNA-binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus., Dell G, Wilkinson KW, Tranter R, Parish J, Leo Brady R, Gaston K, J Mol Biol. 2003 Dec 12;334(5):979-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14643661 14643661]
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[[Category: Human papillomavirus type 6a]]
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[[Category: Human papillomavirus type 1a]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Brady RL]]
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[[Category: Brady, R.L.]]
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[[Category: Dell G]]
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[[Category: Dell, G.]]
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[[Category: Gaston K]]
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[[Category: Gaston, K.]]
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[[Category: Parish J]]
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[[Category: Parish, J.]]
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[[Category: Tranter R]]
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[[Category: Tranter, R.]]
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[[Category: Wilkinson KW]]
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[[Category: Wilkinson, K.W.]]
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[[Category: PO4]]
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[[Category: anti-parallel beta-barrel]]
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[[Category: dna-binding domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:21:09 2007''
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Current revision

Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus

PDB ID 1r8h

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