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1gwm

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[[Image:1gwm.jpg|left|200px]]<br /><applet load="1gwm" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gwm, resolution 1.15&Aring;" />
 
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'''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH GLUCOHEXAOSE'''<br />
 
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==Overview==
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==Carbohydrate binding module family29 complexed with glucohexaose==
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Carbohydrate-protein recognition is central to many biological processes. Enzymes that act on polysaccharide substrates frequently contain noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target the enzyme to the appropriate substrate. CBMs that recognize specific plant structural polysaccharides are often able to accommodate both the variable backbone and the side-chain decorations of heterogeneous ligands. "CBM29" modules, derived from a noncatalytic component of the Piromyces equi cellulase/hemicellulase complex, provide an example of this selective yet flexible recognition. They discriminate strongly against some polysaccharides while remaining relatively promiscuous toward both beta-1,4-linked manno- and cello-oligosaccharides. This feature may reflect preferential, but flexible, targeting toward glucomannans in the plant cell wall. The three-dimensional structure of CBM29-2 and its complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of the aromatic residues complements the conformation of the target sugar polymer while accommodation of both manno- and gluco-configured oligo- and polysaccharides is conferred by virtue of the plasticity of the direct interactions from their axial and equatorial 2-hydroxyls, respectively. Such flexible ligand recognition targets the anaerobic fungal complex to a range of different components in the plant cell wall and thus plays a pivotal role in the highly efficient degradation of this composite structure by the microbial eukaryote.
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<StructureSection load='1gwm' size='340' side='right'caption='[[1gwm]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gwm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_sp._'equi' Piromyces sp. 'equi']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWM FirstGlance]. <br>
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1GWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi] with <scene name='pdbligand=CO:'>CO</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Edo+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWM OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwm OCA], [https://pdbe.org/1gwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwm RCSB], [https://www.ebi.ac.uk/pdbsum/1gwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwm ProSAT]</span></td></tr>
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Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12391332 12391332]
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</table>
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[[Category: Piromyces equi]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q9C171_PIREQ Q9C171_PIREQ]
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[[Category: Charnock, S J.]]
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__TOC__
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[[Category: Davies, G J.]]
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</StructureSection>
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[[Category: Nurizzo, D.]]
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[[Category: Large Structures]]
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[[Category: CO]]
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[[Category: Piromyces sp. 'equi']]
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[[Category: EDO]]
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[[Category: Charnock SJ]]
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[[Category: carbohydrate binding domain]]
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[[Category: Davies GJ]]
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[[Category: cellohexaose]]
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[[Category: Nurizzo D]]
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[[Category: cellulosome]]
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[[Category: glucomannan]]
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[[Category: mannohexaose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:49 2008''
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Current revision

Carbohydrate binding module family29 complexed with glucohexaose

PDB ID 1gwm

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