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1pfs

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==SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE==
==SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE==
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<StructureSection load='1pfs' size='340' side='right' caption='[[1pfs]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
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<StructureSection load='1pfs' size='340' side='right'caption='[[1pfs]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1pfs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf3 Pseudomonas phage pf3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PFS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1pfs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_Pf3 Pseudomonas phage Pf3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pfs RCSB], [http://www.ebi.ac.uk/pdbsum/1pfs PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<table>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfs OCA], [https://pdbe.org/1pfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfs RCSB], [https://www.ebi.ac.uk/pdbsum/1pfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfs ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
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</table>
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== Publication Abstract from PubMed ==
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== Function ==
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The three-dimensional structure of the homodimeric single-stranded DNA binding protein encoded by the filamentous Pseudomonas bacteriophage Pf3 has been determined using heteronuclear multidimensional NMR techniques and restrained molecular dynamics. NMR experiments and structure calculations have been performed on a mutant protein (Phe36 --&gt; His) that was successfully designed to reduce the tendency of the protein to aggregate. The protein monomer is composed of a five-stranded antiparallel beta-sheet from which two beta-hairpins and a large loop protrude. The structure is compared with the single-stranded DNA binding protein encoded by the filamentous Escherichia coli phage Ff, a protein with a similar biological function and DNA binding properties, yet quite different amino acid sequence, and with the major cold shock protein of Escherichia coli, a single-stranded DNA binding protein with an entirely different sequence, biological function and binding characteristics. The amino acid sequence of the latter is highly homologous to the nucleic acid binding domain (i.e. the cold shock domain) of proteins belonging to the Y-box family. Despite their differences in amino acid sequence and function, the folds of the three proteins are remarkably similar, suggesting that this is a preferred folding pattern shared by many single-stranded DNA binding proteins.
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[https://www.uniprot.org/uniprot/G5P_BPPF3 G5P_BPPF3] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
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Solution structure of the single-stranded DNA binding protein of the filamentous Pseudomonas phage Pf3: similarity to other proteins binding to single-stranded nucleic acids.,Folmer RH, Nilges M, Konings RN, Hilbers CW EMBO J. 1995 Sep 1;14(17):4132-42. PMID:7556054<ref>PMID:7556054</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseudomonas phage pf3]]
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[[Category: Large Structures]]
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[[Category: Folmer, R H.A.]]
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[[Category: Pseudomonas phage Pf3]]
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[[Category: Hilbers, C W.]]
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[[Category: Folmer RHA]]
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[[Category: Konings, R N.H.]]
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[[Category: Hilbers CW]]
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[[Category: Nilges, M.]]
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[[Category: Konings RNH]]
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[[Category: Bacteriophage pf3]]
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[[Category: Nilges M]]
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[[Category: Dna-binding protein]]
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[[Category: Single-stranded dna]]
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[[Category: Viral]]
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Current revision

SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE

PDB ID 1pfs

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