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| - | [[Image:1sap.gif|left|200px]] | |
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| - | {{Structure
| + | ==HYPERTHERMOPHILE PROTEIN, RELAXATION MATRIX REFINEMENT STRUCTURE== |
| - | |PDB= 1sap |SIZE=350|CAPTION= <scene name='initialview01'>1sap</scene>
| + | <StructureSection load='1sap' size='340' side='right'caption='[[1sap]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[1sap]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SAP FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE= | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sap OCA], [https://pdbe.org/1sap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sap RCSB], [https://www.ebi.ac.uk/pdbsum/1sap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sap ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sap OCA], [http://www.ebi.ac.uk/pdbsum/1sap PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sap RCSB]</span>
| + | [https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990] |
| - | }}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | '''HYPERTHERMOPHILE PROTEIN, RELAXATION MATRIX REFINEMENT STRUCTURE'''
| + | [[Category: Large Structures]] |
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| - | | + | |
| - | ==Overview==
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| - | The Sac7 proteins from the hyperthermophile Sulfolobus acidocaldarius are a heterogeneous mixture of small, thermostable, nonspecific DNA-binding proteins. One of these proteins, Sac7d, has been overexpressed in Escherichia coli to provide a homogeneous preparation for structure, stability, and function studies. We present here essentially complete sequence-specific 1H NMR assignments for Sac7d, a delineation of secondary structural elements, and the high-resolution solution structure obtained from a full relaxation matrix refinement. The final structure provides an excellent fit to the NMR data with an NOE R-factor of 0.27 for backbone NOEs. The structure has a compact globular fold with 82% of the sequence involved in regular secondary structure: an antiparallel two-stranded beta-ribbon with a tight turn, followed by a short 3(10) helix, an antiparallel three-stranded beta-sheet, another short 3(10) helix, and finally four turns of alpha-helix. The amphipathic alpha-helix packs across the hydrophobic face of the three-stranded beta-sheet in an open-faced sandwich arrangement with at least one turn of the helix exposed beyond the sheet. The hydrophobic face of the beta-ribbon packs against a corner of the twisted beta-sheet. The single tryptophan responsible for the 88% fluorescence quenching upon DNA binding is exposed on the surface of the three-stranded beta-sheet. Lysines 5 and 7, whose monomethylation may be associated with enhanced thermostability, are highly solvent exposed along the inner edge of the two-stranded ribbon. The structure of Sac7d differs in many respects from that reported for the homologous native Sso7d [Baumann et al. (1994) Nature Struct. Biol. 1, 808] with a backbone RMSD greater than 3.0 A, largely due to the packing and length of the C-terminal alpha-helix which may be important in Sac7d DNA binding.
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| - | ==About this Structure==
| + | |
| - | 1SAP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAP OCA].
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| - | ==Reference== | + | |
| - | Solution structure of the DNA-binding protein Sac7d from the hyperthermophile Sulfolobus acidocaldarius., Edmondson SP, Qiu L, Shriver JW, Biochemistry. 1995 Oct 17;34(41):13289-304. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7577913 7577913]
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| - | [[Category: Single protein]] | + | |
| | [[Category: Sulfolobus acidocaldarius]] | | [[Category: Sulfolobus acidocaldarius]] |
| - | [[Category: Edmondson, S P.]] | + | [[Category: Edmondson SP]] |
| - | [[Category: Shriver, J W.]] | + | [[Category: Shriver JW]] |
| - | [[Category: dna-binding protein]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:37 2008''
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