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2bhw

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[[Image:2bhw.gif|left|200px]]<br /><applet load="2bhw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bhw, resolution 2.5&Aring;" />
 
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'''PEA LIGHT-HARVESTING COMPLEX II AT 2.5 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==PEA LIGHT-HARVESTING COMPLEX II AT 2.5 ANGSTROM RESOLUTION==
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The plant light-harvesting complex of photosystem II (LHC-II) collects and, transmits solar energy for photosynthesis in chloroplast membranes and has, essential roles in regulation of photosynthesis and in photoprotection., The 2.5 A structure of pea LHC-II determined by X-ray crystallography of, stacked two-dimensional crystals shows how membranes interact to form, chloroplast grana, and reveals the mutual arrangement of 42 chlorophylls a, and b, 12 carotenoids and six lipids in the LHC-II trimer. Spectral, assignment of individual chlorophylls indicates the flow of energy in the, complex and the mechanism of photoprotection in two close chlorophyll, a-lutein pairs. We propose a simple mechanism for the xanthophyll-related, slow component of nonphotochemical quenching in LHC-II, by which excess, energy is transferred to a zeaxanthin replacing violaxanthin in its, binding site, and dissipated as heat. Our structure shows the complex in a, quenched state, which may be relevant for the rapid, pH-induced component, of nonphotochemical quenching.
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<StructureSection load='2bhw' size='340' side='right'caption='[[2bhw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bhw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHW FirstGlance]. <br>
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2BHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with LUX, NEX, XAT, CLA, CHL, LHG and DGD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Dgd Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHL:CHLOROPHYLL+B'>CHL</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LUX:(3R,3R,6S,9R,9R,13R,13S)-4,5-DIDEHYDRO-5,6,7,8,9,9,10,10,11,11,12,12,13,13,14,14,15,15-OCTADECAHYDRO-BETA,BETA-CAROTENE-3,3-DIOL'>LUX</scene>, <scene name='pdbligand=NEX:(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL'>NEX</scene>, <scene name='pdbligand=XAT:(3S,5R,6S,3S,5R,6S)-5,6,5,6-DIEPOXY-5,6,5,6-+TETRAHYDRO-BETA,BETA-CAROTENE-3,3-DIOL'>XAT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bhw OCA], [https://pdbe.org/2bhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bhw RCSB], [https://www.ebi.ac.uk/pdbsum/2bhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bhw ProSAT]</span></td></tr>
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Mechanisms of photoprotection and nonphotochemical quenching in pea light-harvesting complex at 2.5 A resolution., Standfuss J, Terwisscha van Scheltinga AC, Lamborghini M, Kuhlbrandt W, EMBO J. 2005 Mar 9;24(5):919-28. Epub 2005 Feb 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15719016 15719016]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CB22_PEA CB22_PEA] The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.<ref>PMID:2174365</ref> May channel protons produced in the catalytic Mn center of water oxidation into the thylakoid lumen.<ref>PMID:2174365</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bhw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bhw ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
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[[Category: Single protein]]
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[[Category: Kuehlbrandt W]]
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[[Category: Kuehlbrandt, W.]]
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[[Category: Lamborghini M]]
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[[Category: Lamborghini, M.]]
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[[Category: Standfuss J]]
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[[Category: Scheltinga, A.C.Terwisscha.Van.]]
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[[Category: Terwisscha van Scheltinga AC]]
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[[Category: Standfuss, J.]]
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[[Category: CHL]]
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[[Category: CLA]]
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[[Category: DGD]]
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[[Category: LHG]]
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[[Category: LUX]]
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[[Category: NEX]]
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[[Category: XAT]]
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[[Category: chlorophyll]]
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[[Category: chloroplast]]
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[[Category: chromophore]]
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[[Category: lhc-ii]]
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[[Category: light-harvesting]]
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[[Category: membrane]]
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[[Category: membrane protein]]
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[[Category: phosphorylation]]
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[[Category: photosynthesis]]
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[[Category: photosystem i]]
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[[Category: photosystem ii]]
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[[Category: plastid]]
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[[Category: thylakoid]]
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[[Category: transit peptide]]
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[[Category: transmembrane]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:47:34 2007''
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Current revision

PEA LIGHT-HARVESTING COMPLEX II AT 2.5 ANGSTROM RESOLUTION

PDB ID 2bhw

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