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2bm5

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[[Image:2bm5.gif|left|200px]]<br />
 
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<applet load="2bm5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bm5, resolution 2.0&Aring;" />
 
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'''THE STRUCTURE OF MFPA (RV3361C, P21 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.'''<br />
 
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==Overview==
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==The Structure of MfpA (Rv3361c, P21 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.==
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Fluoroquinolones are gaining increasing importance in the treatment of, tuberculosis. The expression of MfpA, a member of the pentapeptide repeat, family of proteins from Mycobacterium tuberculosis, causes resistance to, ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and, inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that, exhibits size, shape, and electrostatic similarity to B-form DNA. This, represents a form of DNA mimicry and explains both its inhibitory effect, on DNA gyrase and fluoroquinolone resistance resulting from the protein's, expression in vivo.
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<StructureSection load='2bm5' size='340' side='right'caption='[[2bm5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bm5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BM5 FirstGlance]. <br>
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2BM5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BM5 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bm5 OCA], [https://pdbe.org/2bm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bm5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bm5 ProSAT]</span></td></tr>
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A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15933203 15933203]
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</table>
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[[Category: Mycobacterium tuberculosis]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/O50390_MYCTO O50390_MYCTO]
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[[Category: Blanchard, J.S.]]
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== Evolutionary Conservation ==
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[[Category: Hegde, S.S.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Maxwell, A.]]
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Check<jmol>
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[[Category: Mitchenall, L.A.]]
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<jmolCheckbox>
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[[Category: Roderick, S.L.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bm5_consurf.spt"</scriptWhenChecked>
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[[Category: Takiff, H.E.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Vetting, M.W.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: SO4]]
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</jmolCheckbox>
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[[Category: dna gyrase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bm5 ConSurf].
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[[Category: dna mimicry]]
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<div style="clear:both"></div>
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[[Category: fluroquinolone resistance]]
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__TOC__
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[[Category: pentapeptide repeat protein]]
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</StructureSection>
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[[Category: right-handed quadrilateral beta-helix]]
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:42:16 2007''
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[[Category: Blanchard JS]]
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[[Category: Hegde SS]]
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[[Category: Maxwell A]]
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[[Category: Mitchenall LA]]
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[[Category: Roderick SL]]
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[[Category: Takiff HE]]
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[[Category: Vetting MW]]

Current revision

The Structure of MfpA (Rv3361c, P21 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.

PDB ID 2bm5

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