This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2c6u

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:38, 1 May 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2c6u.gif|left|200px]]
 
-
{{Structure
+
==Crystal structure of human CLEC-2 (CLEC1B)==
-
|PDB= 2c6u |SIZE=350|CAPTION= <scene name='initialview01'>2c6u</scene>, resolution 1.60&Aring;
+
<StructureSection load='2c6u' size='340' side='right'caption='[[2c6u]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[2c6u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C6U FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c6u OCA], [https://pdbe.org/2c6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c6u RCSB], [https://www.ebi.ac.uk/pdbsum/2c6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c6u ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
== Function ==
-
'''CRYSTAL STRUCTURE OF HUMAN CLEC-2 (CLEC1B)'''
+
[https://www.uniprot.org/uniprot/CLC1B_HUMAN CLC1B_HUMAN]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
The human C-type lectin-like molecule CLEC-2 is expressed on the surface of platelets and signaling through CLEC-2 causes platelet activation and aggregation. CLEC-2 is a receptor for the platelet-aggregating snake venom protein rhodocytin. It is also a newly identified co-receptor for human immunodeficiency virus type 1 (HIV-1). An endogenous ligand has not yet been identified. We have solved the crystal structure of the extracellular domain of CLEC-2 to 1.6-A resolution, and identified the key structural features involved in ligand binding. A semi-helical loop region and flanking residues dominate the surface that is available for ligand binding. The precise distribution of hydrophobic and electrostatic features in this loop will determine the nature of any endogenous ligand with which it can interact. Major ligand-induced conformational change in CLEC-2 is unlikely as its overall fold is compact and robust. However, ligand binding could induce a tilt of a 3-10 helical portion of the long loop region. Mutational analysis and surface plasmon resonance binding studies support these observations. This study provides a framework for understanding the effects of rhodocytin venom binding on CLEC-2 and for understanding the nature of likely endogenous ligands and will provide a basis for rational design of drugs to block ligand binding.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c6/2c6u_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
2C6U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C6U OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c6u ConSurf].
-
The crystal structure and mutational binding analysis of the extracellular domain of the platelet-activating receptor CLEC-2., Watson AA, Brown J, Harlos K, Eble JA, Walter TS, O'Callaghan CA, J Biol Chem. 2007 Feb 2;282(5):3165-72. Epub 2006 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17132623 17132623]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Brown, J.]]
+
[[Category: Brown J]]
-
[[Category: Callaghan, C A.O.]]
+
[[Category: O'Callaghan CA]]
-
[[Category: Watson, A A.]]
+
[[Category: Watson AA]]
-
[[Category: aggretin]]
+
-
[[Category: c-type lectin-like]]
+
-
[[Category: clec-2]]
+
-
[[Category: clec1b]]
+
-
[[Category: platelet]]
+
-
[[Category: rhodocytin]]
+
-
[[Category: thrombosis]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:11:35 2008''
+

Current revision

Crystal structure of human CLEC-2 (CLEC1B)

PDB ID 2c6u

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c6u"
Personal tools